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• W2010626652 abstract "The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes." @default.
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• W2010626652 date "2009-10-31" @default.
• W2010626652 modified "2023-09-24" @default.
• W2010626652 title "Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells" @default.
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• W2010626652 doi "https://doi.org/10.5483/bmbrep.2009.42.10.691" @default.
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