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- W2010633246 abstract "Glycoinositol phospholipid (GPI) glycans obtained by treatment of purified GPI anchors with 50% aqueous hydrofluoric acid can be permethylated to give a quaternary ammonium ion at the hexosamine, which results in a substantial increase in sensitivity for subsequent analysis by soft-ionization mass spectrometry. Tandem mass spectrometric analysis of these derivatives shows predictable fragmentation patterns that reveal the order of carbohydrate moieties and their branching patterns. Informative tandem mass spectra can be obtained from only 30 pmol of the derivatized glycan. The application of this method to the GPI glycan purified from the scrapie prion protein indicates the presence of structures that differ from other GPI anchors." @default.
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- W2010633246 date "1990-12-01" @default.
- W2010633246 modified "2023-09-24" @default.
- W2010633246 title "Structure determination of glycoinositol phospholipid anchors by permethylation and tandem mass spectrometry" @default.
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- W2010633246 doi "https://doi.org/10.1016/s1046-2023(05)80332-9" @default.
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