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- W2010642016 abstract "Summary We evaluated by nested PCR reaction, different cow secretions from a herd with 48% of prevalence of bovine tuberculosis (BTB), seeking to determine niches where Mycobacterium bovis could be found. Postmortem examination of 18 (75%) tuberculin reacting cows allowed demonstrates BTB‐compatible lesions in six, all of them PCR positives in milk and four in colostra samples. Our results showed that up to 62% of the colostra analysed contained M. bovis DNA, whereas only 18% of milk gave a positive reaction. Moreover, in bronchoalveolar lavages from cattle with compatible lesions in lungs or lymph nodes, where macrophages account up to 90% of cells, we did not find evidences of M. bovis . Altogether, these results suggest that differences in the anti‐bacterial capacity of bovine macrophages, dependent upon microenvironment and organ‐specific factors, exist. Alternatively, we hypothesize that hypoxic conditions that are encountered in mammary glands macrophages could induce M. bovis entrance into a ‘dormancy‐like’ state, and that the high number of colostra samples were M. bovis was detected, could be an indicator of reactivation during ‘peripartum’." @default.
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- W2010642016 date "2008-05-01" @default.
- W2010642016 modified "2023-10-15" @default.
- W2010642016 title "High Frequency of Mycobacterium bovis DNA in Colostra from Tuberculous Cattle Detected by Nested PCR" @default.
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- W2010642016 doi "https://doi.org/10.1111/j.1863-2378.2008.01125.x" @default.
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