FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010689715> ?p ?o ?g. }

• W2010689715 endingPage "5781" @default.
• W2010689715 startingPage "5770" @default.
• W2010689715 abstract "Proteins modified by 4-hydroxy-2-nonenal (HNE) are cellular markers of oxidative stress in health and disease. HNE is generated by free radical chain reactions during oxidative stress as a major end-product of the oxidative fatty acid metabolism. Identification and quantitative analysis of HNE-modified proteins are readily performed by using specific antibodies raised against them. Further on, the identification of the amino acid residues involved in the HNE-modification is an additional step in proteomic post-transcriptional modification analysis to explain the nature of the specificity underlying oxidative stress mechanisms. For this purpose, a combined protocol of immune-detection, peptide enrichment, mass spectrometry, and de novo protein sequencing has been developed. The methodology was first examined in the model protein bovine serum albumin (BSA), allowing the comparison of matrix-assisted laser desorption/ionization-tandem time of flight (MALDI-TOF/TOF) mass spectrometry and liquid chromatography-tandem mass spectrometry (LC-MS/MS) performance and sensitivity. Peptide enrichment was optimized by affinity chromatography on HNE-BSA resulting in increased sensitivity. Identification of amino acid residues modified by HNE was finally ascertained by de novo sequencing analysis. The improved methodology was demonstrated on human erythrocyte membrane proteins allowing the identification of HNE-lysine and HNE-histidine Michael adducts in the β-spectrin under physiological conditions." @default.
• W2010689715 created "2016-06-24" @default.
• W2010689715 creator A5003434750 @default.
• W2010689715 creator A5009471545 @default.
• W2010689715 creator A5035604336 @default.
• W2010689715 creator A5088742599 @default.
• W2010689715 creator A5089658007 @default.
• W2010689715 date "2010-10-08" @default.
• W2010689715 modified "2023-10-18" @default.
• W2010689715 title "Combined Proteomic Approaches for the Identification of Specific Amino Acid Residues Modified by 4-Hydroxy-2-Nonenal under Physiological Conditions" @default.
• W2010689715 cites W1876993892 @default.
• W2010689715 cites W1967103312 @default.
• W2010689715 cites W1970816245 @default.
• W2010689715 cites W1971243875 @default.
• W2010689715 cites W1991366673 @default.
• W2010689715 cites W1994453569 @default.
• W2010689715 cites W1997596252 @default.
• W2010689715 cites W2004778528 @default.
• W2010689715 cites W2007149332 @default.
• W2010689715 cites W2012520364 @default.
• W2010689715 cites W2016953214 @default.
• W2010689715 cites W2017725520 @default.
• W2010689715 cites W2018686718 @default.
• W2010689715 cites W2020193912 @default.
• W2010689715 cites W2022597209 @default.
• W2010689715 cites W2025433424 @default.
• W2010689715 cites W2035013158 @default.
• W2010689715 cites W2038724931 @default.
• W2010689715 cites W2044357091 @default.
• W2010689715 cites W2048623176 @default.
• W2010689715 cites W2049237667 @default.
• W2010689715 cites W2051795211 @default.
• W2010689715 cites W2054824503 @default.
• W2010689715 cites W2058060770 @default.
• W2010689715 cites W2058848674 @default.
• W2010689715 cites W2066270399 @default.
• W2010689715 cites W2071420779 @default.
• W2010689715 cites W2075303826 @default.
• W2010689715 cites W2076273704 @default.
• W2010689715 cites W2080863543 @default.
• W2010689715 cites W2088432481 @default.
• W2010689715 cites W2090531687 @default.
• W2010689715 cites W2094619254 @default.
• W2010689715 cites W2096752298 @default.
• W2010689715 cites W2110966376 @default.
• W2010689715 cites W2129852694 @default.
• W2010689715 cites W2132083914 @default.
• W2010689715 cites W2138560564 @default.
• W2010689715 cites W2142338033 @default.
• W2010689715 cites W2154511372 @default.
• W2010689715 cites W2399056814 @default.
• W2010689715 cites W4205664975 @default.
• W2010689715 doi "https://doi.org/10.1021/pr100555v" @default.
• W2010689715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20818828" @default.
• W2010689715 hasPublicationYear "2010" @default.
• W2010689715 type Work @default.
• W2010689715 sameAs 2010689715 @default.
• W2010689715 citedByCount "24" @default.
• W2010689715 countsByYear W20106897152012 @default.
• W2010689715 countsByYear W20106897152013 @default.
• W2010689715 countsByYear W20106897152014 @default.
• W2010689715 countsByYear W20106897152015 @default.
• W2010689715 countsByYear W20106897152016 @default.
• W2010689715 countsByYear W20106897152017 @default.
• W2010689715 countsByYear W20106897152018 @default.
• W2010689715 countsByYear W20106897152019 @default.
• W2010689715 countsByYear W20106897152020 @default.
• W2010689715 countsByYear W20106897152022 @default.
• W2010689715 countsByYear W20106897152023 @default.
• W2010689715 crossrefType "journal-article" @default.
• W2010689715 hasAuthorship W2010689715A5003434750 @default.
• W2010689715 hasAuthorship W2010689715A5009471545 @default.
• W2010689715 hasAuthorship W2010689715A5035604336 @default.
• W2010689715 hasAuthorship W2010689715A5088742599 @default.
• W2010689715 hasAuthorship W2010689715A5089658007 @default.
• W2010689715 hasConcept C104317684 @default.
• W2010689715 hasConcept C112979427 @default.
• W2010689715 hasConcept C162356407 @default.
• W2010689715 hasConcept C185592680 @default.
• W2010689715 hasConcept C202157981 @default.
• W2010689715 hasConcept C27420735 @default.
• W2010689715 hasConcept C2776151105 @default.
• W2010689715 hasConcept C2779281246 @default.
• W2010689715 hasConcept C31827203 @default.
• W2010689715 hasConcept C43617362 @default.
• W2010689715 hasConcept C46111723 @default.
• W2010689715 hasConcept C515207424 @default.
• W2010689715 hasConcept C55493867 @default.
• W2010689715 hasConceptScore W2010689715C104317684 @default.
• W2010689715 hasConceptScore W2010689715C112979427 @default.
• W2010689715 hasConceptScore W2010689715C162356407 @default.
• W2010689715 hasConceptScore W2010689715C185592680 @default.
• W2010689715 hasConceptScore W2010689715C202157981 @default.
• W2010689715 hasConceptScore W2010689715C27420735 @default.
• W2010689715 hasConceptScore W2010689715C2776151105 @default.
• W2010689715 hasConceptScore W2010689715C2779281246 @default.
• W2010689715 hasConceptScore W2010689715C31827203 @default.
• W2010689715 hasConceptScore W2010689715C43617362 @default.

• next