Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010759957> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2010759957 endingPage "223" @default.
- W2010759957 startingPage "216" @default.
- W2010759957 abstract "Cibacron Blue F3GA was covalently attached onto monosize poly(glycidyl methacrylate) [poly(GMA)] beads for removal of human serum albumin (HSA) from human serum. Monosize poly(GMA) beads, 1.6 μm in diameter, were produced by dispersion polymerization. Cibacron Blue F3GA loading was 1.73 mol/g. HSA adsorption experiments were performed by stirred-batch adsorption. The non-specific adsorption of HSA was low (0.8 mg/g polymer). Dye attachment onto the monosize beads significantly increased the HSA adsorption (189.8 mg/g). The maximum HSA adsorption was observed at pH 5.0. With an increase of the aqueous phase concentration of sodium chloride, the adsorption capacity decreased drastically. The equilibrium adsorption of HSA significantly decreased with increasing temperature. The elution studies were performed by adding 0.1 M Tris/HCl buffer containing 0.5 M NaSCN to the HSA solutions in which adsorption equilibria had been reached. The elution results demonstrated that the adsorption of HSA to the adsorbent was reversible. The depletion efficiencies for HSA were above 87% for all studied concentrations. To test the efficiency of HSA removal from human serum, proteins in the serum and eluted portion were analyzed by two-dimensional gel electrophoresis. Eluted proteins include mainly albumin, and a small number of nonalbumin proteins such as apo-lipoprotein A1, sero-transferrin, haptoglobulin and α1-antitrypsin were bound by the dye-affinity beads. IgA was not identified in eluted fraction." @default.
- W2010759957 created "2016-06-24" @default.
- W2010759957 creator A5000989467 @default.
- W2010759957 creator A5083350052 @default.
- W2010759957 date "2006-03-07" @default.
- W2010759957 modified "2023-10-18" @default.
- W2010759957 title "Efficient removal of albumin from human serum by monosize dye-affinity beads" @default.
- W2010759957 cites W1518739783 @default.
- W2010759957 cites W1542672737 @default.
- W2010759957 cites W1964667730 @default.
- W2010759957 cites W1975366308 @default.
- W2010759957 cites W1987569561 @default.
- W2010759957 cites W1990437884 @default.
- W2010759957 cites W1992730111 @default.
- W2010759957 cites W1994239109 @default.
- W2010759957 cites W2005357459 @default.
- W2010759957 cites W2012426488 @default.
- W2010759957 cites W2021699577 @default.
- W2010759957 cites W2028021708 @default.
- W2010759957 cites W2029918421 @default.
- W2010759957 cites W2036455403 @default.
- W2010759957 cites W2039382479 @default.
- W2010759957 cites W2073396712 @default.
- W2010759957 cites W2076689098 @default.
- W2010759957 cites W2086242301 @default.
- W2010759957 cites W2118747886 @default.
- W2010759957 cites W2128635872 @default.
- W2010759957 cites W68401319 @default.
- W2010759957 doi "https://doi.org/10.1016/j.jchromb.2006.01.006" @default.
- W2010759957 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16464649" @default.
- W2010759957 hasPublicationYear "2006" @default.
- W2010759957 type Work @default.
- W2010759957 sameAs 2010759957 @default.
- W2010759957 citedByCount "76" @default.
- W2010759957 countsByYear W20107599572012 @default.
- W2010759957 countsByYear W20107599572013 @default.
- W2010759957 countsByYear W20107599572014 @default.
- W2010759957 countsByYear W20107599572015 @default.
- W2010759957 countsByYear W20107599572016 @default.
- W2010759957 countsByYear W20107599572018 @default.
- W2010759957 countsByYear W20107599572019 @default.
- W2010759957 crossrefType "journal-article" @default.
- W2010759957 hasAuthorship W2010759957A5000989467 @default.
- W2010759957 hasAuthorship W2010759957A5083350052 @default.
- W2010759957 hasConcept C150394285 @default.
- W2010759957 hasConcept C165616093 @default.
- W2010759957 hasConcept C178790620 @default.
- W2010759957 hasConcept C184651966 @default.
- W2010759957 hasConcept C185592680 @default.
- W2010759957 hasConcept C2776125364 @default.
- W2010759957 hasConcept C2778597219 @default.
- W2010759957 hasConcept C2780801859 @default.
- W2010759957 hasConcept C43617362 @default.
- W2010759957 hasConcept C44228677 @default.
- W2010759957 hasConcept C521977710 @default.
- W2010759957 hasConcept C55493867 @default.
- W2010759957 hasConcept C73826308 @default.
- W2010759957 hasConcept C86807702 @default.
- W2010759957 hasConceptScore W2010759957C150394285 @default.
- W2010759957 hasConceptScore W2010759957C165616093 @default.
- W2010759957 hasConceptScore W2010759957C178790620 @default.
- W2010759957 hasConceptScore W2010759957C184651966 @default.
- W2010759957 hasConceptScore W2010759957C185592680 @default.
- W2010759957 hasConceptScore W2010759957C2776125364 @default.
- W2010759957 hasConceptScore W2010759957C2778597219 @default.
- W2010759957 hasConceptScore W2010759957C2780801859 @default.
- W2010759957 hasConceptScore W2010759957C43617362 @default.
- W2010759957 hasConceptScore W2010759957C44228677 @default.
- W2010759957 hasConceptScore W2010759957C521977710 @default.
- W2010759957 hasConceptScore W2010759957C55493867 @default.
- W2010759957 hasConceptScore W2010759957C73826308 @default.
- W2010759957 hasConceptScore W2010759957C86807702 @default.
- W2010759957 hasIssue "2" @default.
- W2010759957 hasLocation W20107599571 @default.
- W2010759957 hasLocation W20107599572 @default.
- W2010759957 hasOpenAccess W2010759957 @default.
- W2010759957 hasPrimaryLocation W20107599571 @default.
- W2010759957 hasRelatedWork W147684278 @default.
- W2010759957 hasRelatedWork W1554355790 @default.
- W2010759957 hasRelatedWork W1604439525 @default.
- W2010759957 hasRelatedWork W1986226709 @default.
- W2010759957 hasRelatedWork W1990304231 @default.
- W2010759957 hasRelatedWork W2012493618 @default.
- W2010759957 hasRelatedWork W2048971419 @default.
- W2010759957 hasRelatedWork W2051244614 @default.
- W2010759957 hasRelatedWork W2131485520 @default.
- W2010759957 hasRelatedWork W2415885697 @default.
- W2010759957 hasVolume "832" @default.
- W2010759957 isParatext "false" @default.
- W2010759957 isRetracted "false" @default.
- W2010759957 magId "2010759957" @default.
- W2010759957 workType "article" @default.