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- W2010777266 abstract "Abstract The retention characteristics of the major legume seed oligosaccharides (sucrose, raffinose, stachyose and verbascose), and also those of some minor oligosaccharide components of soybeans, lupin seeds and fermented soybean products, were investigated using an RP-18 reversed phase column. Excellent separation of sucrose, raffinose and stachyose from one another and from minor oligosaccharide components was achieved using distilled water as the mobile phase, but verbascose could not be determined separately as it coeluted with raffinose. Addition of salts to the mobile phase increased retention and improved selectivity of separation. Rapid and reasonably efficient separation of sucrose, raffinose, stachyose and verbascose was achieved when 0.3 m ammonium sulphate was used as the mobile phase. These oligosaccharides were clearly resolved from minor oligosaccharide components. A comparison of the retention characteristics of the reversed phase column with those of a continuously modified plain silica column, using an acetonitrile/water/ amine modifier mixture as mobile phase, revealed major differences in selectivity which may be of value for separation and identification purposes." @default.
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- W2010777266 title "Evaluation of a reversed phase high performance liquid chromatographic column for estimation of legume seed oligosaccharides" @default.
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- W2010777266 doi "https://doi.org/10.1016/0308-8146(86)90015-4" @default.
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