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- W2010788613 abstract "The capacity for protein synthesis in protoplasts isolated from hyphae of Aspergillus nidulans during staged lytic digestions was investigated by measurement of uptake and incorporation of [ 14 C]leucine into protoplast samples. All protoplast fractions were active in [ 14 C]leucine uptake but differences in uptake rates and levels of incorporation between different fractions were attributed to protoplast release patterns in the two stabilizer systems used. The effect of suspension of mycelium in buffer/stabilizer solutions of the loss of [ 14 C]leucine from pre-labelled material was investigated to assess the effect of plasmolysis and prolonged suspension in the protoplast isolation medium. Losses of [ 14 C]leucine were a maximum of 22–23% on plasmolysis and prolonged suspension had little further effect; mycelium remained synthetically active under these conditions." @default.
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- W2010788613 title "Protein synthesis in mycelial protoplasts of Aspergillus nidulans" @default.
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- W2010788613 doi "https://doi.org/10.1016/s0007-1536(85)80205-9" @default.
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