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• W2010806463 abstract "Barley seeds were exposed to gamma-irradiation plus caffeine post-treatment under various temperatures (0–30°C) and examined for growth reduction, chromosomal aberrations and chlorophyll mutation. DNA synthesis and release of reducing sugars during the germination of non-irradiated seeds at different temperatures were also determined. The dose-response curve for 7-day-old seedling height was approximated by the standard function, F(D) = 0.9077 [1−(1−e−a1D)5]+0.0923, where F(D) = per cent of control in seedling height, D = radiation exposure, e = base of natural algorithm and a1 = sensitivity coefficient. The extent of repair, expressed as the ratio of the difference between D50 for seedling height with and without caffeine treatment of D50 with irradiation alone, depended on the incubation temperature during the period from 3 hr to 27 hr after soaking. The initiation of sugar-release and 3H-thymidine incorporation was delayed in seeds exposed to 0°C and 10°C; a 30°C-exposure disturbed cellular metabolism and DNA sythesis as evidenced by reductions in sugar-release and 3H-thymidine incorporation. Mutation frequency and spectra after the gamma-ray and caffeine treatment were significantly stimulated by exposure to higher temperatures. It is concluded that thermal potentiation results from the depression of physiological reversion, affecting post-replication repair of gamma-induced lesions, and also that the increase in mutations with temperature is caused by a partial alteration in the conditions inhibiting DNA repair." @default.
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• W2010806463 date "1982-11-01" @default.
• W2010806463 modified "2023-09-26" @default.
• W2010806463 title "Temperature-effect on the repair of gamma-induced lesions in barley seeds" @default.
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• W2010806463 doi "https://doi.org/10.1016/0098-8472(82)90052-1" @default.
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