FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010907801> ?p ?o ?g. }

• W2010907801 endingPage "996" @default.
• W2010907801 startingPage "986" @default.
• W2010907801 abstract "When exposed to stress-provoking environmental conditions such as those of ground waters, many medically important bacteria have been shown to be capable of activating a survival strategy known as the viable but non-culturable (VBNC) state. In this state bacteria are no longer culturable on conventional growth media, but the cells maintain their viability and pathogenicity genes/factors and can start dividing again, in a part of the cell population, upon restoration of favourable environmental conditions. Little is known about the genetic mechanisms underlying the VBNC state. In this study we show evidence of involvement of the rpoS gene in persistence of Escherichia coli in the VBNC state. The kinetics of entry into the non-culturable state and duration of cell viability were measured in two E. coli mutants carrying an inactivated rpoS gene and compared with those of the parents. For these experiments, laboratory microcosms consisting of an artificial oligotrophic medium incubated at 4 degrees C were used. The E. coli parental strains reached the non-culturable state in 33 days when the plate counts were evaluated on Luria-Bertani agar containing sodium pyruvate, whereas cells of the rpoS mutants lost their culturability in only 21 days. Upon reaching unculturability the parents yielded respiring cells and cells with intact membranes for at least the next three weeks and resuscitation was allowed during this time. In contrast, the RpoS- mutant cells demonstrated intact membranes for only two weeks and a very restricted (<7 days) resuscitation capability. Guanosine 3',5'-bispyrophosphate (ppGpp) acts as a positive regulator during the production and functioning of RpoS. A mutant deficient in ppGpp production behaved like the rpoS mutants, while overproducers of ppGpp displayed a vitality at least comparable to that of RpoS+ strains. These results suggest that the E. coli parental strains enter the VBNC state which lasts for, at least, three weeks, after which apparently all the cells die. The rpoS mutants do not activate this survival strategy and early die. This implies involvement of the rpoS gene in E. coli persistence in the VBNC state." @default.
• W2010907801 created "2016-06-24" @default.
• W2010907801 creator A5005017097 @default.
• W2010907801 creator A5031509045 @default.
• W2010907801 creator A5057751904 @default.
• W2010907801 creator A5058517743 @default.
• W2010907801 creator A5075913677 @default.
• W2010907801 date "2003-10-01" @default.
• W2010907801 modified "2023-10-18" @default.
• W2010907801 title "Involvement of rpoS in the survival of Escherichia coli in the viable but non-culturable state" @default.
• W2010907801 cites W1435363487 @default.
• W2010907801 cites W145906557 @default.
• W2010907801 cites W1494346402 @default.
• W2010907801 cites W1536324551 @default.
• W2010907801 cites W1538615579 @default.
• W2010907801 cites W1541808990 @default.
• W2010907801 cites W1583909132 @default.
• W2010907801 cites W1589250908 @default.
• W2010907801 cites W1616230851 @default.
• W2010907801 cites W1658446649 @default.
• W2010907801 cites W18385036 @default.
• W2010907801 cites W1911301314 @default.
• W2010907801 cites W1922471182 @default.
• W2010907801 cites W1973200192 @default.
• W2010907801 cites W1981044242 @default.
• W2010907801 cites W1989074746 @default.
• W2010907801 cites W1993188640 @default.
• W2010907801 cites W1997104297 @default.
• W2010907801 cites W1997580064 @default.
• W2010907801 cites W2009812778 @default.
• W2010907801 cites W2035744376 @default.
• W2010907801 cites W2041628043 @default.
• W2010907801 cites W2042458706 @default.
• W2010907801 cites W2055532796 @default.
• W2010907801 cites W2073988387 @default.
• W2010907801 cites W2079580196 @default.
• W2010907801 cites W2086696634 @default.
• W2010907801 cites W2090406158 @default.
• W2010907801 cites W2101336581 @default.
• W2010907801 cites W2105376921 @default.
• W2010907801 cites W2107109782 @default.
• W2010907801 cites W2121970980 @default.
• W2010907801 cites W2123595055 @default.
• W2010907801 cites W2124786260 @default.
• W2010907801 cites W2124899336 @default.
• W2010907801 cites W2128534098 @default.
• W2010907801 cites W2130502159 @default.
• W2010907801 cites W2135174809 @default.
• W2010907801 cites W2143587535 @default.
• W2010907801 cites W2147006435 @default.
• W2010907801 cites W2157598793 @default.
• W2010907801 cites W2159319229 @default.
• W2010907801 cites W2161737754 @default.
• W2010907801 cites W2164417732 @default.
• W2010907801 cites W2166432249 @default.
• W2010907801 cites W3021526558 @default.
• W2010907801 cites W4213249853 @default.
• W2010907801 cites W53079279 @default.
• W2010907801 doi "https://doi.org/10.1046/j.1462-2920.2003.00497.x" @default.
• W2010907801 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14510852" @default.
• W2010907801 hasPublicationYear "2003" @default.
• W2010907801 type Work @default.
• W2010907801 sameAs 2010907801 @default.
• W2010907801 citedByCount "103" @default.
• W2010907801 countsByYear W20109078012012 @default.
• W2010907801 countsByYear W20109078012013 @default.
• W2010907801 countsByYear W20109078012014 @default.
• W2010907801 countsByYear W20109078012015 @default.
• W2010907801 countsByYear W20109078012016 @default.
• W2010907801 countsByYear W20109078012017 @default.
• W2010907801 countsByYear W20109078012018 @default.
• W2010907801 countsByYear W20109078012019 @default.
• W2010907801 countsByYear W20109078012020 @default.
• W2010907801 countsByYear W20109078012021 @default.
• W2010907801 countsByYear W20109078012022 @default.
• W2010907801 countsByYear W20109078012023 @default.
• W2010907801 crossrefType "journal-article" @default.
• W2010907801 hasAuthorship W2010907801A5005017097 @default.
• W2010907801 hasAuthorship W2010907801A5031509045 @default.
• W2010907801 hasAuthorship W2010907801A5057751904 @default.
• W2010907801 hasAuthorship W2010907801A5058517743 @default.
• W2010907801 hasAuthorship W2010907801A5075913677 @default.
• W2010907801 hasConcept C101762097 @default.
• W2010907801 hasConcept C104317684 @default.
• W2010907801 hasConcept C143065580 @default.
• W2010907801 hasConcept C144024400 @default.
• W2010907801 hasConcept C149923435 @default.
• W2010907801 hasConcept C150194340 @default.
• W2010907801 hasConcept C18903297 @default.
• W2010907801 hasConcept C2776721704 @default.
• W2010907801 hasConcept C2777712861 @default.
• W2010907801 hasConcept C2777995097 @default.
• W2010907801 hasConcept C2778910516 @default.
• W2010907801 hasConcept C2908647359 @default.
• W2010907801 hasConcept C523546767 @default.
• W2010907801 hasConcept C54355233 @default.
• W2010907801 hasConcept C547475151 @default.
• W2010907801 hasConcept C55493867 @default.

• next