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• W2010913202 endingPage "62" @default.
• W2010913202 startingPage "49" @default.
• W2010913202 abstract "Different DNA probes hybridize under different conditions. I examine the constraints of the design of oligonucleotide probes that are meant to hybridize to different unique sites in human genomic DNA under a single set of hybridization conditions as a parallel array. In 522 kb of human genomic DNA, 75% of 12-base and 89% of 22-base are unique, as opposed to 90% and 100% as expected of unstructured DNA, and this is not due solely to repetitive elements in the DNA. Hybridization in TMAC to reduce A + T content effects on melting temperature allows only 90% of unique targets to be hybridized under one set of conditions if a 2°C difference between matched and mismatched sequences is required. Standard hybridization conditions allow no more than 60% of unique probes to be used together. This suggests that probe, hybridization conditions, and instrument design for multiple-probe hybridization applications will be harder than previously suggested." @default.
• W2010913202 created "2016-06-24" @default.
• W2010913202 creator A5004962359 @default.
• W2010913202 date "1994-01-01" @default.
• W2010913202 modified "2023-09-25" @default.
• W2010913202 title "Selection of oligonucleotide probes and experimental conditions for multiplex hybridization experiments" @default.
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• W2010913202 doi "https://doi.org/10.1016/1050-3862(94)90051-5" @default.
• W2010913202 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7803130" @default.
• W2010913202 hasPublicationYear "1994" @default.
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