FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010915029> ?p ?o ?g. }

• W2010915029 endingPage "139" @default.
• W2010915029 startingPage "129" @default.
• W2010915029 abstract "A large number of genome-wide screens using RNA interference (RNAi) libraries have been utilized to determine the function of individual gene products involved in a variety of biological processes. In this study, we describe a new method to enzymatically generate a long hairpin RNA (lhRNA) expression library from a cDNA plasmid library using a nicking endonuclease, BcaBEST DNA polymerase, and Cre recombinase without excising the inserted DNA fragment from the plasmid vector. This method involves 5 steps: (1) conversion of an inserted DNA fragment in a plasmid into a direct repeat (DR); (2) purification of the plasmid containing the DR; (3) subcloning a lox71 cassette into the plasmid; (4) conversion of the DR in the plasmid into an inverted repeat (IR) using Cre recombinase; and (5) purification of the plasmid containing the IR. We also established an efficient method for inserting DNase I-digested DNA fragments into expression plasmids to enable construction of a cDNA plasmid library suitable as source materials to construct the lhRNA expression library. We confirmed that each of the lhRNA expression plasmids constructed using this method induced strong RNAi in a silkworm cell line, NIAS-Bm-oyanagi2." @default.
• W2010915029 created "2016-06-24" @default.
• W2010915029 creator A5043881132 @default.
• W2010915029 creator A5054016490 @default.
• W2010915029 creator A5056244337 @default.
• W2010915029 date "2012-08-01" @default.
• W2010915029 modified "2023-09-26" @default.
• W2010915029 title "Construction of a long hairpin RNA expression library using Cre recombinase" @default.
• W2010915029 cites W1496492558 @default.
• W2010915029 cites W1597411082 @default.
• W2010915029 cites W1647075334 @default.
• W2010915029 cites W1968410036 @default.
• W2010915029 cites W1972937465 @default.
• W2010915029 cites W1973350982 @default.
• W2010915029 cites W1974482866 @default.
• W2010915029 cites W1987776253 @default.
• W2010915029 cites W1992766810 @default.
• W2010915029 cites W1999563094 @default.
• W2010915029 cites W2006745281 @default.
• W2010915029 cites W2038916704 @default.
• W2010915029 cites W2055662724 @default.
• W2010915029 cites W2059558761 @default.
• W2010915029 cites W2091245078 @default.
• W2010915029 cites W2093008096 @default.
• W2010915029 cites W2097659278 @default.
• W2010915029 cites W2107235611 @default.
• W2010915029 cites W2109468839 @default.
• W2010915029 cites W2114406978 @default.
• W2010915029 cites W2118537613 @default.
• W2010915029 cites W2126672666 @default.
• W2010915029 cites W2128946743 @default.
• W2010915029 cites W2146138017 @default.
• W2010915029 cites W2149400066 @default.
• W2010915029 cites W2160543432 @default.
• W2010915029 cites W2164631379 @default.
• W2010915029 cites W4211039187 @default.
• W2010915029 doi "https://doi.org/10.1016/j.jbiotec.2012.03.016" @default.
• W2010915029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22484114" @default.
• W2010915029 hasPublicationYear "2012" @default.
• W2010915029 type Work @default.
• W2010915029 sameAs 2010915029 @default.
• W2010915029 citedByCount "6" @default.
• W2010915029 countsByYear W20109150292012 @default.
• W2010915029 countsByYear W20109150292015 @default.
• W2010915029 countsByYear W20109150292016 @default.
• W2010915029 countsByYear W20109150292021 @default.
• W2010915029 crossrefType "journal-article" @default.
• W2010915029 hasAuthorship W2010915029A5043881132 @default.
• W2010915029 hasAuthorship W2010915029A5054016490 @default.
• W2010915029 hasAuthorship W2010915029A5056244337 @default.
• W2010915029 hasConcept C100012643 @default.
• W2010915029 hasConcept C102414288 @default.
• W2010915029 hasConcept C104317684 @default.
• W2010915029 hasConcept C13575015 @default.
• W2010915029 hasConcept C153911025 @default.
• W2010915029 hasConcept C156695909 @default.
• W2010915029 hasConcept C163005120 @default.
• W2010915029 hasConcept C166703698 @default.
• W2010915029 hasConcept C187882448 @default.
• W2010915029 hasConcept C189482833 @default.
• W2010915029 hasConcept C190232843 @default.
• W2010915029 hasConcept C22744801 @default.
• W2010915029 hasConcept C3017666073 @default.
• W2010915029 hasConcept C40767141 @default.
• W2010915029 hasConcept C54355233 @default.
• W2010915029 hasConcept C552990157 @default.
• W2010915029 hasConcept C67705224 @default.
• W2010915029 hasConcept C86803240 @default.
• W2010915029 hasConcept C92087593 @default.
• W2010915029 hasConceptScore W2010915029C100012643 @default.
• W2010915029 hasConceptScore W2010915029C102414288 @default.
• W2010915029 hasConceptScore W2010915029C104317684 @default.
• W2010915029 hasConceptScore W2010915029C13575015 @default.
• W2010915029 hasConceptScore W2010915029C153911025 @default.
• W2010915029 hasConceptScore W2010915029C156695909 @default.
• W2010915029 hasConceptScore W2010915029C163005120 @default.
• W2010915029 hasConceptScore W2010915029C166703698 @default.
• W2010915029 hasConceptScore W2010915029C187882448 @default.
• W2010915029 hasConceptScore W2010915029C189482833 @default.
• W2010915029 hasConceptScore W2010915029C190232843 @default.
• W2010915029 hasConceptScore W2010915029C22744801 @default.
• W2010915029 hasConceptScore W2010915029C3017666073 @default.
• W2010915029 hasConceptScore W2010915029C40767141 @default.
• W2010915029 hasConceptScore W2010915029C54355233 @default.
• W2010915029 hasConceptScore W2010915029C552990157 @default.
• W2010915029 hasConceptScore W2010915029C67705224 @default.
• W2010915029 hasConceptScore W2010915029C86803240 @default.
• W2010915029 hasConceptScore W2010915029C92087593 @default.
• W2010915029 hasIssue "3-4" @default.
• W2010915029 hasLocation W20109150291 @default.
• W2010915029 hasLocation W20109150292 @default.
• W2010915029 hasOpenAccess W2010915029 @default.
• W2010915029 hasPrimaryLocation W20109150291 @default.
• W2010915029 hasRelatedWork W1730115118 @default.
• W2010915029 hasRelatedWork W1973254433 @default.
• W2010915029 hasRelatedWork W1996686187 @default.
• W2010915029 hasRelatedWork W2007269451 @default.
• W2010915029 hasRelatedWork W2010915029 @default.

• next