FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010924908> ?p ?o ?g. }

• W2010924908 endingPage "43" @default.
• W2010924908 startingPage "33" @default.
• W2010924908 abstract "Macrophages accumulate in the vascular wall during conditions such as hypertension and hypercholesterolemia and contribute to vascular remodelling. Here we describe a method for the isolation and subsequent flow cytometric analysis of macrophages from aortas of mice. Cell suspensions were prepared from thoracic aortas of male C57BL/6J mice by a combination of manual disruption, incubation in enzymatic digestion medium, and passage through a 70 μm cell strainer. Flow cytometric analysis of these suspensions revealed a high content of cells with strong light scattering properties (i.e. SSC(hi)) compared with suspensions derived from mouse blood, spleen, thymus or kidney. Unstained aortic cell suspensions also displayed a preponderance of autofluorescence in the B670, V560, V460, B525 and V610 channels of the flow cytometer, suggesting that these channels should be avoided for subsequent flow cytometric analyses, at least for initial gating steps. Thus, aortic preparations were labelled with an APC-Cy7-conjugated antibody against the pan-leukocyte marker, CD45, as well as an APC-conjugated antibody against the macrophage-specific antigen, F4/80, as these fluorochromes emit in channels that displayed relatively low levels of auto-fluorescence in our initial studies (i.e. R780 and R660). Flow cytometric analysis of labelled aortic preparations revealed a distinct population of CD45(+)F4/80(+) cells. Importantly, back-gating on this CD45(+)F4/80(+) cell population showed it to be now virtually devoid of autofluorescence in all remaining open channels, and hence an appropriate foundation for further detailed analysis of macrophage polarization using multiple intra- and extra-cellular markers. Furthermore, we demonstrated that angiotensin II-induced hypertension in C57BL6/J mice, and hypercholesterolemia in apolipoprotein E-deficient mice, each resulted in an approximate doubling of CD45(+)F4/80(+) cells in the aortic wall, highlighting the utility of our new protocol for studying the impact of disease on macrophage accumulation in the vascular wall." @default.
• W2010924908 created "2016-06-24" @default.
• W2010924908 creator A5010995435 @default.
• W2010924908 creator A5011831615 @default.
• W2010924908 creator A5038896735 @default.
• W2010924908 creator A5051677621 @default.
• W2010924908 creator A5069893866 @default.
• W2010924908 creator A5082142817 @default.
• W2010924908 creator A5089701670 @default.
• W2010924908 date "2013-10-01" @default.
• W2010924908 modified "2023-09-26" @default.
• W2010924908 title "A flow cytometric method for the analysis of macrophages in the vascular wall" @default.
• W2010924908 cites W110134706 @default.
• W2010924908 cites W1486131515 @default.
• W2010924908 cites W1650965741 @default.
• W2010924908 cites W1970876023 @default.
• W2010924908 cites W1981367181 @default.
• W2010924908 cites W1985859986 @default.
• W2010924908 cites W1995866677 @default.
• W2010924908 cites W1997315475 @default.
• W2010924908 cites W1997887701 @default.
• W2010924908 cites W2012080265 @default.
• W2010924908 cites W2015141049 @default.
• W2010924908 cites W2015163460 @default.
• W2010924908 cites W2033896075 @default.
• W2010924908 cites W2055247780 @default.
• W2010924908 cites W2060578093 @default.
• W2010924908 cites W2066404725 @default.
• W2010924908 cites W2079968028 @default.
• W2010924908 cites W2106478043 @default.
• W2010924908 cites W2117185007 @default.
• W2010924908 cites W2117795062 @default.
• W2010924908 cites W2120061572 @default.
• W2010924908 cites W2137499920 @default.
• W2010924908 cites W2153626991 @default.
• W2010924908 cites W2157615046 @default.
• W2010924908 cites W2158380859 @default.
• W2010924908 cites W2159198711 @default.
• W2010924908 cites W2162405407 @default.
• W2010924908 cites W2166747879 @default.
• W2010924908 doi "https://doi.org/10.1016/j.jim.2013.07.009" @default.
• W2010924908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23928493" @default.
• W2010924908 hasPublicationYear "2013" @default.
• W2010924908 type Work @default.
• W2010924908 sameAs 2010924908 @default.
• W2010924908 citedByCount "13" @default.
• W2010924908 countsByYear W20109249082015 @default.
• W2010924908 countsByYear W20109249082016 @default.
• W2010924908 countsByYear W20109249082018 @default.
• W2010924908 countsByYear W20109249082019 @default.
• W2010924908 countsByYear W20109249082021 @default.
• W2010924908 crossrefType "journal-article" @default.
• W2010924908 hasAuthorship W2010924908A5010995435 @default.
• W2010924908 hasAuthorship W2010924908A5011831615 @default.
• W2010924908 hasAuthorship W2010924908A5038896735 @default.
• W2010924908 hasAuthorship W2010924908A5051677621 @default.
• W2010924908 hasAuthorship W2010924908A5069893866 @default.
• W2010924908 hasAuthorship W2010924908A5082142817 @default.
• W2010924908 hasAuthorship W2010924908A5089701670 @default.
• W2010924908 hasConcept C121332964 @default.
• W2010924908 hasConcept C142724271 @default.
• W2010924908 hasConcept C1491633281 @default.
• W2010924908 hasConcept C153911025 @default.
• W2010924908 hasConcept C159654299 @default.
• W2010924908 hasConcept C179687394 @default.
• W2010924908 hasConcept C185592680 @default.
• W2010924908 hasConcept C202751555 @default.
• W2010924908 hasConcept C203014093 @default.
• W2010924908 hasConcept C2779244956 @default.
• W2010924908 hasConcept C2908647359 @default.
• W2010924908 hasConcept C553184892 @default.
• W2010924908 hasConcept C55493867 @default.
• W2010924908 hasConcept C62520636 @default.
• W2010924908 hasConcept C71924100 @default.
• W2010924908 hasConcept C86803240 @default.
• W2010924908 hasConcept C91881484 @default.
• W2010924908 hasConcept C99454951 @default.
• W2010924908 hasConceptScore W2010924908C121332964 @default.
• W2010924908 hasConceptScore W2010924908C142724271 @default.
• W2010924908 hasConceptScore W2010924908C1491633281 @default.
• W2010924908 hasConceptScore W2010924908C153911025 @default.
• W2010924908 hasConceptScore W2010924908C159654299 @default.
• W2010924908 hasConceptScore W2010924908C179687394 @default.
• W2010924908 hasConceptScore W2010924908C185592680 @default.
• W2010924908 hasConceptScore W2010924908C202751555 @default.
• W2010924908 hasConceptScore W2010924908C203014093 @default.
• W2010924908 hasConceptScore W2010924908C2779244956 @default.
• W2010924908 hasConceptScore W2010924908C2908647359 @default.
• W2010924908 hasConceptScore W2010924908C553184892 @default.
• W2010924908 hasConceptScore W2010924908C55493867 @default.
• W2010924908 hasConceptScore W2010924908C62520636 @default.
• W2010924908 hasConceptScore W2010924908C71924100 @default.
• W2010924908 hasConceptScore W2010924908C86803240 @default.
• W2010924908 hasConceptScore W2010924908C91881484 @default.
• W2010924908 hasConceptScore W2010924908C99454951 @default.
• W2010924908 hasIssue "1-2" @default.
• W2010924908 hasLocation W20109249081 @default.
• W2010924908 hasLocation W20109249082 @default.

• next