FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010929465> ?p ?o ?g. }

• W2010929465 endingPage "216" @default.
• W2010929465 startingPage "210" @default.
• W2010929465 abstract "Background Thiazolidinediones (TZDs) have been demonstrated to possess antitumor effects in breast cancer cells, although the mechanisms are not well established. We sought to better define TZDs' antitumor effects and molecular mechanisms to permit rational utilization of these agents. Materials and methods We studied the effects of TZDs on DNA synthesis (BrdU enzyme-linked immunosorbent assay), gene expression (microarray, quantitative reverse transcription-polymerase chain reaction, and immunoblot), serine phosphorylation and localization of β-catenin (nuclear/cytoplasmic fractionation and immunoblot), transactivation activity of β-catenin/T cell factor 4 (TCF4) (luciferase assay with wild-type and mutant TCF4 responsive element), and β-catenin/TCF4 complex (immunoprecipitation) in human breast cancer cells MDA-MB-231 and T47D. Results Troglitazone (TG) down-regulated DNA synthesis in MDA-MB-231 and T47D and decreased mRNA expression of the Wnt co-receptors frizzled-1 and low-density lipoprotein-related protein 6 (LRP6) as well as protein level of LRP6. TG also targets downstream Wnt signaling molecules in T47D cells. TG down-regulates p-β-catenin (S33/S37/T41) and promotes translocation of β-catenin into the nucleus. However, TG inhibits β-catenin-mediated transactivation by down-regulating TCF4 protein levels, thereby inhibiting β-catenin/TCF4 complex formation. Finally, we found that inhibition of Akt augments TG-mediated down-regulation of DNA synthesis and this result was accompanied with the decreased protein levels of Wnt signaling molecules: LRP6, β-catenin, and TCF4. Conclusion These results suggest that the Wnt/β-catenin signaling pathway, which plays an important role in breast cancer pathogenesis, may be a target for TZD treatment. Moreover, a combination of TZDs and a specific Akt inhibitor may serve as a new approach to target Wnt/β-catenin directly and via PI3K/Akt action on glycogen synthase-3β. Thiazolidinediones (TZDs) have been demonstrated to possess antitumor effects in breast cancer cells, although the mechanisms are not well established. We sought to better define TZDs' antitumor effects and molecular mechanisms to permit rational utilization of these agents. We studied the effects of TZDs on DNA synthesis (BrdU enzyme-linked immunosorbent assay), gene expression (microarray, quantitative reverse transcription-polymerase chain reaction, and immunoblot), serine phosphorylation and localization of β-catenin (nuclear/cytoplasmic fractionation and immunoblot), transactivation activity of β-catenin/T cell factor 4 (TCF4) (luciferase assay with wild-type and mutant TCF4 responsive element), and β-catenin/TCF4 complex (immunoprecipitation) in human breast cancer cells MDA-MB-231 and T47D. Troglitazone (TG) down-regulated DNA synthesis in MDA-MB-231 and T47D and decreased mRNA expression of the Wnt co-receptors frizzled-1 and low-density lipoprotein-related protein 6 (LRP6) as well as protein level of LRP6. TG also targets downstream Wnt signaling molecules in T47D cells. TG down-regulates p-β-catenin (S33/S37/T41) and promotes translocation of β-catenin into the nucleus. However, TG inhibits β-catenin-mediated transactivation by down-regulating TCF4 protein levels, thereby inhibiting β-catenin/TCF4 complex formation. Finally, we found that inhibition of Akt augments TG-mediated down-regulation of DNA synthesis and this result was accompanied with the decreased protein levels of Wnt signaling molecules: LRP6, β-catenin, and TCF4. These results suggest that the Wnt/β-catenin signaling pathway, which plays an important role in breast cancer pathogenesis, may be a target for TZD treatment. Moreover, a combination of TZDs and a specific Akt inhibitor may serve as a new approach to target Wnt/β-catenin directly and via PI3K/Akt action on glycogen synthase-3β." @default.
• W2010929465 created "2016-06-24" @default.
• W2010929465 creator A5004744315 @default.
• W2010929465 creator A5018659406 @default.
• W2010929465 creator A5022041848 @default.
• W2010929465 creator A5022256893 @default.
• W2010929465 creator A5039174329 @default.
• W2010929465 creator A5071579955 @default.
• W2010929465 creator A5074454934 @default.
• W2010929465 date "2009-05-01" @default.
• W2010929465 modified "2023-10-18" @default.
• W2010929465 title "Thiazolidinediones Downregulate Wnt/β-Catenin Signaling Via Multiple Mechanisms in Breast Cancer Cells" @default.
• W2010929465 cites W1554815317 @default.
• W2010929465 cites W1976329877 @default.
• W2010929465 cites W1977094101 @default.
• W2010929465 cites W1982411301 @default.
• W2010929465 cites W1983809085 @default.
• W2010929465 cites W1988656176 @default.
• W2010929465 cites W1990671646 @default.
• W2010929465 cites W1993303817 @default.
• W2010929465 cites W2014134228 @default.
• W2010929465 cites W2019251169 @default.
• W2010929465 cites W2020712616 @default.
• W2010929465 cites W2027652494 @default.
• W2010929465 cites W2033618020 @default.
• W2010929465 cites W2040263952 @default.
• W2010929465 cites W2042828511 @default.
• W2010929465 cites W2054154194 @default.
• W2010929465 cites W2054374001 @default.
• W2010929465 cites W2078172132 @default.
• W2010929465 cites W2086028819 @default.
• W2010929465 cites W2124961982 @default.
• W2010929465 cites W2125344994 @default.
• W2010929465 cites W2148074532 @default.
• W2010929465 cites W2152935821 @default.
• W2010929465 cites W2163938907 @default.
• W2010929465 cites W2170899721 @default.
• W2010929465 doi "https://doi.org/10.1016/j.jss.2008.05.032" @default.
• W2010929465 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18639247" @default.
• W2010929465 hasPublicationYear "2009" @default.
• W2010929465 type Work @default.
• W2010929465 sameAs 2010929465 @default.
• W2010929465 citedByCount "25" @default.
• W2010929465 countsByYear W20109294652012 @default.
• W2010929465 countsByYear W20109294652013 @default.
• W2010929465 countsByYear W20109294652014 @default.
• W2010929465 countsByYear W20109294652016 @default.
• W2010929465 countsByYear W20109294652018 @default.
• W2010929465 countsByYear W20109294652021 @default.
• W2010929465 countsByYear W20109294652022 @default.
• W2010929465 countsByYear W20109294652023 @default.
• W2010929465 crossrefType "journal-article" @default.
• W2010929465 hasAuthorship W2010929465A5004744315 @default.
• W2010929465 hasAuthorship W2010929465A5018659406 @default.
• W2010929465 hasAuthorship W2010929465A5022041848 @default.
• W2010929465 hasAuthorship W2010929465A5022256893 @default.
• W2010929465 hasAuthorship W2010929465A5039174329 @default.
• W2010929465 hasAuthorship W2010929465A5071579955 @default.
• W2010929465 hasAuthorship W2010929465A5074454934 @default.
• W2010929465 hasConcept C104317684 @default.
• W2010929465 hasConcept C107300179 @default.
• W2010929465 hasConcept C1292079 @default.
• W2010929465 hasConcept C137620995 @default.
• W2010929465 hasConcept C153911025 @default.
• W2010929465 hasConcept C170320316 @default.
• W2010929465 hasConcept C185592680 @default.
• W2010929465 hasConcept C2779324961 @default.
• W2010929465 hasConcept C30145163 @default.
• W2010929465 hasConcept C502942594 @default.
• W2010929465 hasConcept C55493867 @default.
• W2010929465 hasConcept C62478195 @default.
• W2010929465 hasConcept C86339819 @default.
• W2010929465 hasConcept C86803240 @default.
• W2010929465 hasConcept C95444343 @default.
• W2010929465 hasConceptScore W2010929465C104317684 @default.
• W2010929465 hasConceptScore W2010929465C107300179 @default.
• W2010929465 hasConceptScore W2010929465C1292079 @default.
• W2010929465 hasConceptScore W2010929465C137620995 @default.
• W2010929465 hasConceptScore W2010929465C153911025 @default.
• W2010929465 hasConceptScore W2010929465C170320316 @default.
• W2010929465 hasConceptScore W2010929465C185592680 @default.
• W2010929465 hasConceptScore W2010929465C2779324961 @default.
• W2010929465 hasConceptScore W2010929465C30145163 @default.
• W2010929465 hasConceptScore W2010929465C502942594 @default.
• W2010929465 hasConceptScore W2010929465C55493867 @default.
• W2010929465 hasConceptScore W2010929465C62478195 @default.
• W2010929465 hasConceptScore W2010929465C86339819 @default.
• W2010929465 hasConceptScore W2010929465C86803240 @default.
• W2010929465 hasConceptScore W2010929465C95444343 @default.
• W2010929465 hasIssue "2" @default.
• W2010929465 hasLocation W20109294651 @default.
• W2010929465 hasLocation W20109294652 @default.
• W2010929465 hasOpenAccess W2010929465 @default.
• W2010929465 hasPrimaryLocation W20109294651 @default.
• W2010929465 hasRelatedWork W1964589725 @default.
• W2010929465 hasRelatedWork W1974942829 @default.
• W2010929465 hasRelatedWork W1984644937 @default.
• W2010929465 hasRelatedWork W2038556753 @default.

• next