FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010974860> ?p ?o ?g. }

• W2010974860 endingPage "427" @default.
• W2010974860 startingPage "420" @default.
• W2010974860 abstract "Rationale: New vaccine approaches are needed for Pseudomonas aeruginosa, which continues to be a major cause of serious pulmonary infections. Although Th17 cells can protect against gram-negative pathogens at mucosal surfaces, including the lung, the bacterial proteins recognized by Th17 cells are largely unknown and could be potential new vaccine candidates.Objectives: We describe a strategy to identify Th17-stimulating protein antigens of Pseudomonas aeruginosa to assess their efficacy as vaccines against pneumonia.Methods: Using a library of in vitro transcribed and translated P. aeruginosa proteins, we screened for Th17-stimulating antigens by coculturing the library proteins with splenocytes from mice immunized with a live-attenuated P. aeruginosa vaccine that is protective via Th17-based immunity. We measured antibody and Th17 responses after intranasal immunization of mice with the purified proteins mixed with the Th17 adjuvant curdlan, and we tested the protective efficacy of vaccination in a murine model of acute pneumonia.Measurements and Main Results: The proteins PopB, FpvA, FptA, OprL, and PilQ elicited strong IL-17 secretion in the screen, and purified versions of PopB, FpvA, and OprL stimulated high IL-17 production from immune splenocytes. Immunization with PopB, which is a highly conserved component of the type III secretion system and a known virulence factor, elicited Th17 responses and also enhanced clearance of P. aeruginosa from the lung and spleen after challenge. PopB-immunized mice were protected from lethal pneumonia in an antibody-independent, IL-17–dependent manner.Conclusions: Screening for Th17-stimulating protein antigens identified PopB as a novel and promising vaccine candidate for P. aeruginosa." @default.
• W2010974860 created "2016-06-24" @default.
• W2010974860 creator A5010698345 @default.
• W2010974860 creator A5034471224 @default.
• W2010974860 creator A5048573353 @default.
• W2010974860 creator A5052424282 @default.
• W2010974860 creator A5053422448 @default.
• W2010974860 creator A5055739238 @default.
• W2010974860 creator A5062382858 @default.
• W2010974860 creator A5075028017 @default.
• W2010974860 date "2012-09-01" @default.
• W2010974860 modified "2023-09-24" @default.
• W2010974860 title "Th17-stimulating Protein Vaccines Confer Protection against <i>Pseudomonas aeruginosa</i> Pneumonia" @default.
• W2010974860 cites W1511786918 @default.
• W2010974860 cites W1593914605 @default.
• W2010974860 cites W1936419178 @default.
• W2010974860 cites W1964465182 @default.
• W2010974860 cites W1966714629 @default.
• W2010974860 cites W1975426885 @default.
• W2010974860 cites W1982118335 @default.
• W2010974860 cites W1996122986 @default.
• W2010974860 cites W1996395105 @default.
• W2010974860 cites W1996770717 @default.
• W2010974860 cites W2003961381 @default.
• W2010974860 cites W2005570679 @default.
• W2010974860 cites W2013584442 @default.
• W2010974860 cites W2014338056 @default.
• W2010974860 cites W2015763512 @default.
• W2010974860 cites W2017321063 @default.
• W2010974860 cites W2019228627 @default.
• W2010974860 cites W2025866713 @default.
• W2010974860 cites W2028819914 @default.
• W2010974860 cites W2039739211 @default.
• W2010974860 cites W2041543177 @default.
• W2010974860 cites W2056186055 @default.
• W2010974860 cites W2062106725 @default.
• W2010974860 cites W2067906280 @default.
• W2010974860 cites W2085705181 @default.
• W2010974860 cites W2097898346 @default.
• W2010974860 cites W2100990607 @default.
• W2010974860 cites W2109623890 @default.
• W2010974860 cites W2112133235 @default.
• W2010974860 cites W2124672930 @default.
• W2010974860 cites W2125397987 @default.
• W2010974860 cites W2128101553 @default.
• W2010974860 cites W2128721558 @default.
• W2010974860 cites W2132938540 @default.
• W2010974860 cites W2139049032 @default.
• W2010974860 cites W2139939070 @default.
• W2010974860 cites W2142717815 @default.
• W2010974860 cites W2143532882 @default.
• W2010974860 cites W2146250612 @default.
• W2010974860 cites W2147826635 @default.
• W2010974860 cites W2149885115 @default.
• W2010974860 cites W2154337891 @default.
• W2010974860 cites W2157600770 @default.
• W2010974860 cites W2160883911 @default.
• W2010974860 cites W2161014327 @default.
• W2010974860 cites W2164719325 @default.
• W2010974860 cites W2169241573 @default.
• W2010974860 cites W4210274142 @default.
• W2010974860 doi "https://doi.org/10.1164/rccm.201202-0182oc" @default.
• W2010974860 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3443805" @default.
• W2010974860 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22723292" @default.
• W2010974860 hasPublicationYear "2012" @default.
• W2010974860 type Work @default.
• W2010974860 sameAs 2010974860 @default.
• W2010974860 citedByCount "112" @default.
• W2010974860 countsByYear W20109748602012 @default.
• W2010974860 countsByYear W20109748602013 @default.
• W2010974860 countsByYear W20109748602014 @default.
• W2010974860 countsByYear W20109748602015 @default.
• W2010974860 countsByYear W20109748602016 @default.
• W2010974860 countsByYear W20109748602017 @default.
• W2010974860 countsByYear W20109748602018 @default.
• W2010974860 countsByYear W20109748602019 @default.
• W2010974860 countsByYear W20109748602020 @default.
• W2010974860 countsByYear W20109748602021 @default.
• W2010974860 countsByYear W20109748602022 @default.
• W2010974860 countsByYear W20109748602023 @default.
• W2010974860 crossrefType "journal-article" @default.
• W2010974860 hasAuthorship W2010974860A5010698345 @default.
• W2010974860 hasAuthorship W2010974860A5034471224 @default.
• W2010974860 hasAuthorship W2010974860A5048573353 @default.
• W2010974860 hasAuthorship W2010974860A5052424282 @default.
• W2010974860 hasAuthorship W2010974860A5053422448 @default.
• W2010974860 hasAuthorship W2010974860A5055739238 @default.
• W2010974860 hasAuthorship W2010974860A5062382858 @default.
• W2010974860 hasAuthorship W2010974860A5075028017 @default.
• W2010974860 hasBestOaLocation W20109748602 @default.
• W2010974860 hasConcept C147483822 @default.
• W2010974860 hasConcept C189976730 @default.
• W2010974860 hasConcept C203014093 @default.
• W2010974860 hasConcept C22070199 @default.
• W2010974860 hasConcept C2777637488 @default.
• W2010974860 hasConcept C2777863537 @default.
• W2010974860 hasConcept C2780801004 @default.
• W2010974860 hasConcept C523546767 @default.

• next