FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2010994037> ?p ?o ?g. }

• W2010994037 endingPage "5171" @default.
• W2010994037 startingPage "5167" @default.
• W2010994037 abstract "The Bacillus licheniformis and Bacillus amyloliquefaciens subtilisins differ by 31% in protein sequence and by factors of greater than 60 in catalytic efficiency, kcat/Km, toward various substrates. Despite large differences in sequence and substrate specificity for these serine proteases, only two amino acid substitutions (residues 156 and 217) occur within 4 A (contact distance) of modeled substrates, and a third substitution (residue 169) is within 7 A. The three B. licheniformis substitutions (Ser-156/Ala-169/Leu-217) were introduced into the wild-type B. amyloliquefaciens subtilisin (Glu-156/Gly-169/Tyr-217) by site-directed mutagenesis. The substrate specificity of the triple mutant approaches that of B. licheniformis enzyme when assayed with seven different substrates that vary in charge, size, and hydrophobicity. Thus, specificity properties of distantly related and functionally divergent enzymes can be exchanged by limited amino acid replacements, in this case representing less than 4% of the sequence differences." @default.
• W2010994037 created "2016-06-24" @default.
• W2010994037 creator A5008277017 @default.
• W2010994037 creator A5008303766 @default.
• W2010994037 creator A5055441920 @default.
• W2010994037 creator A5067812218 @default.
• W2010994037 date "1987-08-01" @default.
• W2010994037 modified "2023-10-14" @default.
• W2010994037 title "Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering." @default.
• W2010994037 cites W1474551401 @default.
• W2010994037 cites W153791503 @default.
• W2010994037 cites W1541008516 @default.
• W2010994037 cites W1544045128 @default.
• W2010994037 cites W1554616255 @default.
• W2010994037 cites W1588598829 @default.
• W2010994037 cites W1590376353 @default.
• W2010994037 cites W1601425978 @default.
• W2010994037 cites W1911747505 @default.
• W2010994037 cites W1959540130 @default.
• W2010994037 cites W1965018976 @default.
• W2010994037 cites W1969400276 @default.
• W2010994037 cites W1974988689 @default.
• W2010994037 cites W1977076531 @default.
• W2010994037 cites W1978754461 @default.
• W2010994037 cites W1979212500 @default.
• W2010994037 cites W1985469117 @default.
• W2010994037 cites W2007833676 @default.
• W2010994037 cites W2018093699 @default.
• W2010994037 cites W2024421359 @default.
• W2010994037 cites W2025450446 @default.
• W2010994037 cites W2034708139 @default.
• W2010994037 cites W2043855562 @default.
• W2010994037 cites W2062595820 @default.
• W2010994037 cites W2066448033 @default.
• W2010994037 cites W2067133968 @default.
• W2010994037 cites W2072948774 @default.
• W2010994037 cites W2075097399 @default.
• W2010994037 cites W2092002806 @default.
• W2010994037 cites W2095249666 @default.
• W2010994037 cites W2099474689 @default.
• W2010994037 cites W2138270253 @default.
• W2010994037 cites W2145368473 @default.
• W2010994037 doi "https://doi.org/10.1073/pnas.84.15.5167" @default.
• W2010994037 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/298815" @default.
• W2010994037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3299378" @default.
• W2010994037 hasPublicationYear "1987" @default.
• W2010994037 type Work @default.
• W2010994037 sameAs 2010994037 @default.
• W2010994037 citedByCount "120" @default.
• W2010994037 countsByYear W20109940372012 @default.
• W2010994037 countsByYear W20109940372013 @default.
• W2010994037 countsByYear W20109940372015 @default.
• W2010994037 countsByYear W20109940372016 @default.
• W2010994037 countsByYear W20109940372017 @default.
• W2010994037 countsByYear W20109940372019 @default.
• W2010994037 countsByYear W20109940372020 @default.
• W2010994037 countsByYear W20109940372021 @default.
• W2010994037 countsByYear W20109940372023 @default.
• W2010994037 crossrefType "journal-article" @default.
• W2010994037 hasAuthorship W2010994037A5008277017 @default.
• W2010994037 hasAuthorship W2010994037A5008303766 @default.
• W2010994037 hasAuthorship W2010994037A5055441920 @default.
• W2010994037 hasAuthorship W2010994037A5067812218 @default.
• W2010994037 hasBestOaLocation W20109940371 @default.
• W2010994037 hasConcept C100544194 @default.
• W2010994037 hasConcept C103408237 @default.
• W2010994037 hasConcept C104317684 @default.
• W2010994037 hasConcept C143065580 @default.
• W2010994037 hasConcept C147816474 @default.
• W2010994037 hasConcept C167625842 @default.
• W2010994037 hasConcept C181199279 @default.
• W2010994037 hasConcept C182220744 @default.
• W2010994037 hasConcept C18903297 @default.
• W2010994037 hasConcept C2776414213 @default.
• W2010994037 hasConcept C2776527165 @default.
• W2010994037 hasConcept C2777272437 @default.
• W2010994037 hasConcept C2777289219 @default.
• W2010994037 hasConcept C2777811704 @default.
• W2010994037 hasConcept C2779811187 @default.
• W2010994037 hasConcept C2781338088 @default.
• W2010994037 hasConcept C41183919 @default.
• W2010994037 hasConcept C515207424 @default.
• W2010994037 hasConcept C523546767 @default.
• W2010994037 hasConcept C54355233 @default.
• W2010994037 hasConcept C55493867 @default.
• W2010994037 hasConcept C56856141 @default.
• W2010994037 hasConcept C86803240 @default.
• W2010994037 hasConceptScore W2010994037C100544194 @default.
• W2010994037 hasConceptScore W2010994037C103408237 @default.
• W2010994037 hasConceptScore W2010994037C104317684 @default.
• W2010994037 hasConceptScore W2010994037C143065580 @default.
• W2010994037 hasConceptScore W2010994037C147816474 @default.
• W2010994037 hasConceptScore W2010994037C167625842 @default.
• W2010994037 hasConceptScore W2010994037C181199279 @default.
• W2010994037 hasConceptScore W2010994037C182220744 @default.
• W2010994037 hasConceptScore W2010994037C18903297 @default.
• W2010994037 hasConceptScore W2010994037C2776414213 @default.
• W2010994037 hasConceptScore W2010994037C2776527165 @default.

• next