FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011027148> ?p ?o ?g. }

• W2011027148 endingPage "278" @default.
• W2011027148 startingPage "267" @default.
• W2011027148 abstract "Non-invasive techniques to monitor stress hormones in small animals like mice offer several advantages and are highly demanded in laboratory as well as in field research. Since knowledge about the species-specific metabolism and excretion of glucocorticoids is essential to develop such a technique, we conducted radiometabolism studies in mice (Mus musculus f. domesticus, strain C57BL/6J). Each mouse was injected intraperitoneally with 740 kBq of 3H-labelled corticosterone and all voided urine and fecal samples were collected for five days. In a first experiment 16 animals (eight of each sex) received the injection at 9 a.m., while eight mice (four of each sex) were injected at 9 p.m. in a second experiment. In both experiments radioactive metabolites were recovered predominantly in the feces, although males excreted significantly higher proportions via the feces (about 73%) than females (about 53%). Peak radioactivity in the urine was detected within about 2 h after injection, while in the feces peak concentrations were observed later (depending on the time of injection: about 10 h postinjection in experiment 1 and about 4 h postinjection in experiment 2, thus proving an effect of the time of day). The number and relative abundance of fecal [3H]corticosterone metabolites was determined by high performance liquid chromatography (HPLC). The HPLC separations revealed that corticosterone was extensively metabolized mainly to more polar substances. Regarding the types of metabolites formed, significant differences were found between males and females, but not between the experiments. Additionally, the immunoreactivity of these metabolites was assessed by screening the HPLC fractions with four enzyme immunoassays (EIA). However, only a newly established EIA for 5α-pregnane-3β,11β,21-triol-20-one (measuring corticosterone metabolites with a 5α-3β,11β-diol structure) detected several peaks of radioactive metabolites with high intensity in both sexes, while the other EIAs showed only minor immunoreactivity. Thus, our study for the first time provides substantial information about metabolism and excretion of corticosterone in urine and feces of mice and is the first demonstrating a significant impact of the animals’ sex and the time of day. Based on these data it should be possible to monitor adrenocortical activity non-invasively in this species by measuring fecal corticosterone metabolites with the newly developed EIA. Since mice are extensively used in research world-wide, this could open new perspectives in various fields from ecology to behavioral endocrinology." @default.
• W2011027148 created "2016-06-24" @default.
• W2011027148 creator A5024313897 @default.
• W2011027148 creator A5027895389 @default.
• W2011027148 creator A5046036187 @default.
• W2011027148 creator A5061223077 @default.
• W2011027148 date "2003-02-01" @default.
• W2011027148 modified "2023-10-18" @default.
• W2011027148 title "Effects of sex and time of day on metabolism and excretion of corticosterone in urine and feces of mice" @default.
• W2011027148 cites W110169556 @default.
• W2011027148 cites W1493562038 @default.
• W2011027148 cites W1571688997 @default.
• W2011027148 cites W1635246077 @default.
• W2011027148 cites W168739068 @default.
• W2011027148 cites W1964775268 @default.
• W2011027148 cites W1969694110 @default.
• W2011027148 cites W1972853535 @default.
• W2011027148 cites W1974177346 @default.
• W2011027148 cites W1975869940 @default.
• W2011027148 cites W1979218266 @default.
• W2011027148 cites W1981960003 @default.
• W2011027148 cites W1991947120 @default.
• W2011027148 cites W2012425759 @default.
• W2011027148 cites W2030369194 @default.
• W2011027148 cites W2036674007 @default.
• W2011027148 cites W2039381569 @default.
• W2011027148 cites W2045607854 @default.
• W2011027148 cites W2051508062 @default.
• W2011027148 cites W2065676386 @default.
• W2011027148 cites W2065942825 @default.
• W2011027148 cites W2068392981 @default.
• W2011027148 cites W2068725716 @default.
• W2011027148 cites W2069110673 @default.
• W2011027148 cites W2091531784 @default.
• W2011027148 cites W2094677029 @default.
• W2011027148 cites W2100890647 @default.
• W2011027148 cites W2105224505 @default.
• W2011027148 cites W2107152976 @default.
• W2011027148 cites W2108542703 @default.
• W2011027148 cites W2111245372 @default.
• W2011027148 cites W2120481990 @default.
• W2011027148 cites W2134196208 @default.
• W2011027148 cites W2137498056 @default.
• W2011027148 cites W2137796746 @default.
• W2011027148 cites W2155529925 @default.
• W2011027148 cites W2172044114 @default.
• W2011027148 cites W2172187560 @default.
• W2011027148 cites W2297632339 @default.
• W2011027148 cites W2412428721 @default.
• W2011027148 cites W2921503985 @default.
• W2011027148 doi "https://doi.org/10.1016/s0016-6480(02)00620-2" @default.
• W2011027148 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12606269" @default.
• W2011027148 hasPublicationYear "2003" @default.
• W2011027148 type Work @default.
• W2011027148 sameAs 2011027148 @default.
• W2011027148 citedByCount "485" @default.
• W2011027148 countsByYear W20110271482012 @default.
• W2011027148 countsByYear W20110271482013 @default.
• W2011027148 countsByYear W20110271482014 @default.
• W2011027148 countsByYear W20110271482015 @default.
• W2011027148 countsByYear W20110271482016 @default.
• W2011027148 countsByYear W20110271482017 @default.
• W2011027148 countsByYear W20110271482018 @default.
• W2011027148 countsByYear W20110271482019 @default.
• W2011027148 countsByYear W20110271482020 @default.
• W2011027148 countsByYear W20110271482021 @default.
• W2011027148 countsByYear W20110271482022 @default.
• W2011027148 countsByYear W20110271482023 @default.
• W2011027148 crossrefType "journal-article" @default.
• W2011027148 hasAuthorship W2011027148A5024313897 @default.
• W2011027148 hasAuthorship W2011027148A5027895389 @default.
• W2011027148 hasAuthorship W2011027148A5046036187 @default.
• W2011027148 hasAuthorship W2011027148A5061223077 @default.
• W2011027148 hasConcept C10146269 @default.
• W2011027148 hasConcept C104488531 @default.
• W2011027148 hasConcept C126322002 @default.
• W2011027148 hasConcept C134018914 @default.
• W2011027148 hasConcept C179998833 @default.
• W2011027148 hasConcept C185592680 @default.
• W2011027148 hasConcept C2777477808 @default.
• W2011027148 hasConcept C2780026642 @default.
• W2011027148 hasConcept C2780352252 @default.
• W2011027148 hasConcept C43617362 @default.
• W2011027148 hasConcept C54355233 @default.
• W2011027148 hasConcept C61716771 @default.
• W2011027148 hasConcept C62231903 @default.
• W2011027148 hasConcept C71315377 @default.
• W2011027148 hasConcept C71924100 @default.
• W2011027148 hasConcept C86803240 @default.
• W2011027148 hasConcept C89423630 @default.
• W2011027148 hasConceptScore W2011027148C10146269 @default.
• W2011027148 hasConceptScore W2011027148C104488531 @default.
• W2011027148 hasConceptScore W2011027148C126322002 @default.
• W2011027148 hasConceptScore W2011027148C134018914 @default.
• W2011027148 hasConceptScore W2011027148C179998833 @default.
• W2011027148 hasConceptScore W2011027148C185592680 @default.
• W2011027148 hasConceptScore W2011027148C2777477808 @default.
• W2011027148 hasConceptScore W2011027148C2780026642 @default.

• next