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- W2011042030 abstract "Abstract A new approach is suggested for studying changes in the interactions of protein with DNA in the cells. Measurements of the buoyant density of chromatin were performed in somatic cells and in cells undergoing meiosis in fish. During the process of spermatogenesis some of the somatic histones on the DNA are replaced by a new class of proteins; consequently, the mature sperm contains a unique type of protein having a low mol. wt and a high proportion of arginine. The chromatin obtained from mature sperm is composed of a single component with a density of 1.48–1.49 g/cm3 as measured by CsCl equilibrium sedimentation. On the other hand, somatic cells contain chromatin with lower densities. Chromatin obtained from erythrocytes contains a single component with a density of 1.41–1.42 g/cm3 while liver chromatin shows two components; a main component with a density of 1.45–1.46 and a more heterogeneous component with a lighter density (1.32–1.35). There is a correlation between the buoyant density of the chromatin, the type of its basic proteins and the level of biosynthetic activity in the cells." @default.
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- W2011042030 date "1974-01-01" @default.
- W2011042030 modified "2023-09-27" @default.
- W2011042030 title "Fractionation of somatic and sperm chromatin during spermatogenesis in fish" @default.
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- W2011042030 doi "https://doi.org/10.1016/0014-4827(74)90685-5" @default.
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