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• W2011053260 abstract "1 More than 75% of the RNA polymerase activity of isolated nuclei from human placental tissue was found in the supernatant (20 min at 40000Xg) after incubation of the nuclear suspension for 24 hours at 0°, followed by 15 min at 37° and a further 15 min at 37° in the presence of 120 mM ammonium sulphate. 2 Chromatography of this soluble preparation on DEAE cellulose (0.64 mequiv./g) was carried out using a linear gradient from 100 to 500 mM ammonium sulphate. RNA polymerase activity, eluted at 300 mM ammonium sulphate, was fully dependent on added DNA template. 3 Some properties of human RNA polymerase at various stages of purification are described. 4 The process of solubilization is discussed." @default.
• W2011053260 created "2016-06-24" @default.
• W2011053260 creator A5082920254 @default.
• W2011053260 date "1969-06-01" @default.
• W2011053260 modified "2023-09-25" @default.
• W2011053260 title "Purification and Some Properties of a Soluble DNA-Dependent RNA Polymerase from Nuclei of Human Placenta" @default.
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• W2011053260 doi "https://doi.org/10.1111/j.1432-1033.1969.tb00610.x" @default.
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