FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011057428> ?p ?o ?g. }

• W2011057428 endingPage "2688" @default.
• W2011057428 startingPage "2678" @default.
• W2011057428 abstract "Pertussis toxin (PT) is used in its formaldehyde-detoxified form in acellular pertussis vaccines for preventing whooping cough in children. The effects of formaldehyde treatment (up to 0.5% (w/v) formaldehyde) on the size, molecular association, folding and monoclonal antibody (mAb) binding of PT were studied to further define the structural nature of the high molecular weight species as related to their epitope integrity. Analytical ultracentrifugation (AUC) demonstrated that formaldehyde treatment of PT prevented the dissociation of the holotoxin. Together with results from size exclusion chromatography (SEC), SEC/multi-angle laser light scattering (MALLS) and immunoblotting it was demonstrated that PT increased in molecular weight and heterogeneity as a function of formaldehyde concentration, caused at least in part by covalent cross-linking. Five mAbs specific for PT subunits (S1-S5) bound to the cross-linked species, although there was some loss of epitopes in the larger aggregates. Intrinsic fluorescence spectroscopy gave evidence of progressive unfolding and re-association of PT. These findings demonstrate that a favourable balance between protein stabilisation and denaturation may be achieved by the treatment of pertussis toxin with formaldehyde, and provides a basis for determining the significance of high molecular weight cross-linked species of pertussis toxin in protection against whooping cough." @default.
• W2011057428 created "2016-06-24" @default.
• W2011057428 creator A5020650768 @default.
• W2011057428 creator A5045611603 @default.
• W2011057428 creator A5046635626 @default.
• W2011057428 creator A5061104692 @default.
• W2011057428 creator A5062404711 @default.
• W2011057428 creator A5085839548 @default.
• W2011057428 date "2003-06-01" @default.
• W2011057428 modified "2023-09-26" @default.
• W2011057428 title "Novel configurations of high molecular weight species of the pertussis toxin vaccine component" @default.
• W2011057428 cites W1587585765 @default.
• W2011057428 cites W1754421332 @default.
• W2011057428 cites W1913723649 @default.
• W2011057428 cites W1941562760 @default.
• W2011057428 cites W1976963291 @default.
• W2011057428 cites W1981061882 @default.
• W2011057428 cites W1982445341 @default.
• W2011057428 cites W1996529121 @default.
• W2011057428 cites W1998876456 @default.
• W2011057428 cites W2001332558 @default.
• W2011057428 cites W2024908512 @default.
• W2011057428 cites W2044971646 @default.
• W2011057428 cites W2048750828 @default.
• W2011057428 cites W2056176279 @default.
• W2011057428 cites W2061294847 @default.
• W2011057428 cites W2068362958 @default.
• W2011057428 cites W2081926721 @default.
• W2011057428 cites W2090685385 @default.
• W2011057428 cites W2104572249 @default.
• W2011057428 cites W2105118935 @default.
• W2011057428 cites W2110680433 @default.
• W2011057428 cites W2118814125 @default.
• W2011057428 cites W2128938457 @default.
• W2011057428 cites W2131007317 @default.
• W2011057428 cites W2132617216 @default.
• W2011057428 cites W2135961954 @default.
• W2011057428 cites W2143172175 @default.
• W2011057428 cites W2152385842 @default.
• W2011057428 cites W2158401596 @default.
• W2011057428 cites W2161281144 @default.
• W2011057428 cites W2280710667 @default.
• W2011057428 cites W2413104159 @default.
• W2011057428 cites W2489866311 @default.
• W2011057428 doi "https://doi.org/10.1016/s0264-410x(03)00105-1" @default.
• W2011057428 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12744905" @default.
• W2011057428 hasPublicationYear "2003" @default.
• W2011057428 type Work @default.
• W2011057428 sameAs 2011057428 @default.
• W2011057428 citedByCount "16" @default.
• W2011057428 countsByYear W20110574282012 @default.
• W2011057428 countsByYear W20110574282013 @default.
• W2011057428 countsByYear W20110574282016 @default.
• W2011057428 countsByYear W20110574282019 @default.
• W2011057428 countsByYear W20110574282021 @default.
• W2011057428 crossrefType "journal-article" @default.
• W2011057428 hasAuthorship W2011057428A5020650768 @default.
• W2011057428 hasAuthorship W2011057428A5045611603 @default.
• W2011057428 hasAuthorship W2011057428A5046635626 @default.
• W2011057428 hasAuthorship W2011057428A5061104692 @default.
• W2011057428 hasAuthorship W2011057428A5062404711 @default.
• W2011057428 hasAuthorship W2011057428A5085839548 @default.
• W2011057428 hasConcept C13965031 @default.
• W2011057428 hasConcept C159047783 @default.
• W2011057428 hasConcept C159654299 @default.
• W2011057428 hasConcept C170493617 @default.
• W2011057428 hasConcept C181199279 @default.
• W2011057428 hasConcept C185592680 @default.
• W2011057428 hasConcept C193363816 @default.
• W2011057428 hasConcept C195616568 @default.
• W2011057428 hasConcept C203014093 @default.
• W2011057428 hasConcept C22070199 @default.
• W2011057428 hasConcept C2775930285 @default.
• W2011057428 hasConcept C2776923230 @default.
• W2011057428 hasConcept C2777367657 @default.
• W2011057428 hasConcept C2779214499 @default.
• W2011057428 hasConcept C49773422 @default.
• W2011057428 hasConcept C542903549 @default.
• W2011057428 hasConcept C55493867 @default.
• W2011057428 hasConcept C80631254 @default.
• W2011057428 hasConcept C86803240 @default.
• W2011057428 hasConceptScore W2011057428C13965031 @default.
• W2011057428 hasConceptScore W2011057428C159047783 @default.
• W2011057428 hasConceptScore W2011057428C159654299 @default.
• W2011057428 hasConceptScore W2011057428C170493617 @default.
• W2011057428 hasConceptScore W2011057428C181199279 @default.
• W2011057428 hasConceptScore W2011057428C185592680 @default.
• W2011057428 hasConceptScore W2011057428C193363816 @default.
• W2011057428 hasConceptScore W2011057428C195616568 @default.
• W2011057428 hasConceptScore W2011057428C203014093 @default.
• W2011057428 hasConceptScore W2011057428C22070199 @default.
• W2011057428 hasConceptScore W2011057428C2775930285 @default.
• W2011057428 hasConceptScore W2011057428C2776923230 @default.
• W2011057428 hasConceptScore W2011057428C2777367657 @default.
• W2011057428 hasConceptScore W2011057428C2779214499 @default.
• W2011057428 hasConceptScore W2011057428C49773422 @default.
• W2011057428 hasConceptScore W2011057428C542903549 @default.
• W2011057428 hasConceptScore W2011057428C55493867 @default.

• next