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- W2011064838 abstract "Immunocytochemical observations with purified antibodies revealed the presence of met-enkephalin-like material in the primitive nerve cell line F7 derived from the fetal mouse hypothalamus and previously shown to synthesize somatostatin (1). Radioimmunoassays associated with Bio-gel P 2 chromatography confirmed that met-enkephalin itself accounted (at least partly) for the positive immunocytochemical reaction. Trypsinization (± carboxypeptidase B treatment) of cell extracts significantly increased their met-enkephalin-like immunoreactivity therefore suggesting that met-enkephalin precursors were also present in the F7 clone. Parallel studies on the hypothalamus of fetal mice indicated that met-enkephalin but apparently no precursor was already present at embryonic day 15. The clonal cells F7 may be an appropriate model for investigating the functional correlate of the co-occurrence of metenkephalin and somatostatin in the same cells." @default.
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- W2011064838 date "1982-07-01" @default.
- W2011064838 modified "2023-09-27" @default.
- W2011064838 title "Presence of met-enkephalin in a somatostatin-synthesizing cell line derived from the fetal mouse hypothalamus" @default.
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- W2011064838 doi "https://doi.org/10.1016/0143-4179(82)90072-5" @default.
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