Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011103685> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W2011103685 endingPage "460" @default.
- W2011103685 startingPage "455" @default.
- W2011103685 abstract "Transketolase (EC 2.2.1.1) was purified 12-fold from an extract of dried Candida utilis by two fractionations with acetone followed by chromatography on DEAE-Sephadex. The fractions from the column were free from D-ribose 5-phosphate ketol isomerase and D-ribulose 5-phosphate 3-epimerase and could be used for the assay of D-xylulose 5-phosphate and of the epimerase. Three peaks of transketolase activity were eluted from the chromatography column; the material from each peak had a Michaelis constant for D-xylulose 5-phosphate close to the mean value for the three peaks of 6.8 × 10 −5 M and gave the same two-banded pattern on cellulose acetate electrophoresis. When transaldolase was present in the material placed on the column, it appeared with transketolase in each of the three peaks. The enzyme could be stored for several months, in solution frozen at −20°. Dialysis against water caused a loss of activity which could be restored by the addition of thiamine pyrophosphate. The enzyme catalyzed the condensation of hydroxypyruvate with D-glyceraldehyde phosphate to form D-xylulose 5-phosphate." @default.
- W2011103685 created "2016-06-24" @default.
- W2011103685 creator A5010599616 @default.
- W2011103685 creator A5022394297 @default.
- W2011103685 creator A5060260440 @default.
- W2011103685 date "1969-04-01" @default.
- W2011103685 modified "2023-09-26" @default.
- W2011103685 title "The purification of transketolase from <i>Candida utilis</i>" @default.
- W2011103685 doi "https://doi.org/10.1139/o69-071" @default.
- W2011103685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5769093" @default.
- W2011103685 hasPublicationYear "1969" @default.
- W2011103685 type Work @default.
- W2011103685 sameAs 2011103685 @default.
- W2011103685 citedByCount "28" @default.
- W2011103685 crossrefType "journal-article" @default.
- W2011103685 hasAuthorship W2011103685A5010599616 @default.
- W2011103685 hasAuthorship W2011103685A5022394297 @default.
- W2011103685 hasAuthorship W2011103685A5060260440 @default.
- W2011103685 hasConcept C103432212 @default.
- W2011103685 hasConcept C163994747 @default.
- W2011103685 hasConcept C181199279 @default.
- W2011103685 hasConcept C185592680 @default.
- W2011103685 hasConcept C197957613 @default.
- W2011103685 hasConcept C2777132085 @default.
- W2011103685 hasConcept C2780047943 @default.
- W2011103685 hasConcept C43617362 @default.
- W2011103685 hasConcept C55493867 @default.
- W2011103685 hasConceptScore W2011103685C103432212 @default.
- W2011103685 hasConceptScore W2011103685C163994747 @default.
- W2011103685 hasConceptScore W2011103685C181199279 @default.
- W2011103685 hasConceptScore W2011103685C185592680 @default.
- W2011103685 hasConceptScore W2011103685C197957613 @default.
- W2011103685 hasConceptScore W2011103685C2777132085 @default.
- W2011103685 hasConceptScore W2011103685C2780047943 @default.
- W2011103685 hasConceptScore W2011103685C43617362 @default.
- W2011103685 hasConceptScore W2011103685C55493867 @default.
- W2011103685 hasIssue "4" @default.
- W2011103685 hasLocation W20111036851 @default.
- W2011103685 hasLocation W20111036852 @default.
- W2011103685 hasOpenAccess W2011103685 @default.
- W2011103685 hasPrimaryLocation W20111036851 @default.
- W2011103685 hasRelatedWork W1971308409 @default.
- W2011103685 hasRelatedWork W1985334975 @default.
- W2011103685 hasRelatedWork W1990846267 @default.
- W2011103685 hasRelatedWork W2024775214 @default.
- W2011103685 hasRelatedWork W2040106523 @default.
- W2011103685 hasRelatedWork W2065781312 @default.
- W2011103685 hasRelatedWork W2159559088 @default.
- W2011103685 hasRelatedWork W2208020336 @default.
- W2011103685 hasRelatedWork W2407728773 @default.
- W2011103685 hasRelatedWork W4250471400 @default.
- W2011103685 hasVolume "47" @default.
- W2011103685 isParatext "false" @default.
- W2011103685 isRetracted "false" @default.
- W2011103685 magId "2011103685" @default.
- W2011103685 workType "article" @default.