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- W2011121031 abstract "When novel sequences are isolated by differential display and other methods, it seems useful to determine which is a sense sequence at an early stage before further experiments. A novel sequence, named MT-001, which shows enhanced expression in the permanent ischemic rat brain, was isolated by differential display. Based on this sequence, a primer set for both direction was designed. Each primer was used to make a cDNA and PCRs performed with each cDNA and both primers. One primer used in the RT step produced a PCR product at the expected position, but another primer in the reverse direction could not. This result indicated that the primer that made the expected PCR product is antisense." @default.
- W2011121031 created "2016-06-24" @default.
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- W2011121031 date "2003-01-01" @default.
- W2011121031 modified "2023-10-17" @default.
- W2011121031 title "Determination method of sense sequence based on RT/PCR" @default.
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- W2011121031 doi "https://doi.org/10.1016/s0165-022x(02)00176-8" @default.
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