Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011124105> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2011124105 abstract "Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, ILBackground: We have previously identified a cyclic peptide PLZ4 through screening of “one bead one compound”(OBOC), that specifically binds to bladder urothelial carcinoma cells. The main objective of this study is: 1) to optimize the binding and diagnostic potential of PLZ4 on cancer cells in urine, 2) to determine the imaging potential in xenograft mouse model. Methods: PLZ4 peptide was conjugated to the fluorescent dye Alexa 488 to determine the binding affinity toward several human and canine clinical biopsy smears and paraffin-fixed bladder tumor sections. It was also tested for in vitro diagnostic efficiency with human and dog bladder cancer cell lines treated with urine for 2 and 4 hours. To determine the in vivo targeting and imaging efficacy, PLZ4 was labeled with 64Cu through the chelator DOTA or near-infrared dye DiD loaded in nanocarriers. Mice carrying subcutaneous human or dog bladder cancer xenografts generated from cell lines or clinical specimens were used for in vivo imaging. Results: Our results indicated that PLZ4-Alexa 488 bound to primary bladder cancer obtained from dog and human patients and had higher affinity in paraffin-fixed bladder tumor sections compared to H232A lung cancer xenograft tissue sections. PLZ4 also retained its affinity toward both human and dog bladder cancer cells in the presence of urine for 2 and 4 hours. Positron emission tomography (PET) imaging displayed the accumulation of PLZ4-DOTA-64Cu conjugate at human xenograft bladder cancer sites, while liver and kidney were also found to have high uptake. DiD-loaded nanomicelles decorated with PLZ4 showed a better homing property than non-targeting nanomicelles in both human and canine xenograft bladder cancers. Conclusion: PLZ4 has high imaging, diagnostic and therapeutic potential both in vitro and in vivo. It can possibly be developed for targeted delivery of radionuclides or chemotherapeutic nanoformulations in the management of bladder cancer.Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 367. doi:1538-7445.AM2012-367" @default.
- W2011124105 created "2016-06-24" @default.
- W2011124105 creator A5016546967 @default.
- W2011124105 creator A5017220491 @default.
- W2011124105 creator A5055864540 @default.
- W2011124105 creator A5065066504 @default.
- W2011124105 creator A5080694061 @default.
- W2011124105 date "2012-04-15" @default.
- W2011124105 modified "2023-09-25" @default.
- W2011124105 title "Abstract 367: Imaging and diagnostic potentials of a novel cancer targeting peptide on bladder cancerin vitroandin vivo" @default.
- W2011124105 doi "https://doi.org/10.1158/1538-7445.am2012-367" @default.
- W2011124105 hasPublicationYear "2012" @default.
- W2011124105 type Work @default.
- W2011124105 sameAs 2011124105 @default.
- W2011124105 citedByCount "0" @default.
- W2011124105 crossrefType "proceedings-article" @default.
- W2011124105 hasAuthorship W2011124105A5016546967 @default.
- W2011124105 hasAuthorship W2011124105A5017220491 @default.
- W2011124105 hasAuthorship W2011124105A5055864540 @default.
- W2011124105 hasAuthorship W2011124105A5065066504 @default.
- W2011124105 hasAuthorship W2011124105A5080694061 @default.
- W2011124105 hasConcept C121608353 @default.
- W2011124105 hasConcept C126322002 @default.
- W2011124105 hasConcept C142724271 @default.
- W2011124105 hasConcept C150903083 @default.
- W2011124105 hasConcept C18150654 @default.
- W2011124105 hasConcept C204787440 @default.
- W2011124105 hasConcept C207001950 @default.
- W2011124105 hasConcept C2778172261 @default.
- W2011124105 hasConcept C2778769751 @default.
- W2011124105 hasConcept C2780035454 @default.
- W2011124105 hasConcept C2780352672 @default.
- W2011124105 hasConcept C29331040 @default.
- W2011124105 hasConcept C502942594 @default.
- W2011124105 hasConcept C71924100 @default.
- W2011124105 hasConcept C86803240 @default.
- W2011124105 hasConcept C96232424 @default.
- W2011124105 hasConcept C98274493 @default.
- W2011124105 hasConceptScore W2011124105C121608353 @default.
- W2011124105 hasConceptScore W2011124105C126322002 @default.
- W2011124105 hasConceptScore W2011124105C142724271 @default.
- W2011124105 hasConceptScore W2011124105C150903083 @default.
- W2011124105 hasConceptScore W2011124105C18150654 @default.
- W2011124105 hasConceptScore W2011124105C204787440 @default.
- W2011124105 hasConceptScore W2011124105C207001950 @default.
- W2011124105 hasConceptScore W2011124105C2778172261 @default.
- W2011124105 hasConceptScore W2011124105C2778769751 @default.
- W2011124105 hasConceptScore W2011124105C2780035454 @default.
- W2011124105 hasConceptScore W2011124105C2780352672 @default.
- W2011124105 hasConceptScore W2011124105C29331040 @default.
- W2011124105 hasConceptScore W2011124105C502942594 @default.
- W2011124105 hasConceptScore W2011124105C71924100 @default.
- W2011124105 hasConceptScore W2011124105C86803240 @default.
- W2011124105 hasConceptScore W2011124105C96232424 @default.
- W2011124105 hasConceptScore W2011124105C98274493 @default.
- W2011124105 hasLocation W20111241051 @default.
- W2011124105 hasOpenAccess W2011124105 @default.
- W2011124105 hasPrimaryLocation W20111241051 @default.
- W2011124105 hasRelatedWork W1964518077 @default.
- W2011124105 hasRelatedWork W1967023047 @default.
- W2011124105 hasRelatedWork W1993430800 @default.
- W2011124105 hasRelatedWork W1997220011 @default.
- W2011124105 hasRelatedWork W2035344050 @default.
- W2011124105 hasRelatedWork W2037409533 @default.
- W2011124105 hasRelatedWork W2061339970 @default.
- W2011124105 hasRelatedWork W2099912528 @default.
- W2011124105 hasRelatedWork W2125416432 @default.
- W2011124105 hasRelatedWork W2133805334 @default.
- W2011124105 hasRelatedWork W2133860094 @default.
- W2011124105 hasRelatedWork W2333749640 @default.
- W2011124105 hasRelatedWork W2400338301 @default.
- W2011124105 hasRelatedWork W2410525554 @default.
- W2011124105 hasRelatedWork W2478747245 @default.
- W2011124105 hasRelatedWork W2484330438 @default.
- W2011124105 hasRelatedWork W2494471209 @default.
- W2011124105 hasRelatedWork W2580675833 @default.
- W2011124105 hasRelatedWork W2614729080 @default.
- W2011124105 hasRelatedWork W3026422809 @default.
- W2011124105 isParatext "false" @default.
- W2011124105 isRetracted "false" @default.
- W2011124105 magId "2011124105" @default.
- W2011124105 workType "article" @default.