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- W2011128547 abstract "Prostate glands of young adult mice were grown by the organ culture technique in natural and defined medium, in air and in an atmosphere of 95 per cent O2 and 5 per cent CO2. Under all conditions the alveolar structure of the organ was preserved in vitro. In glands kept in natural medium the alveoli were lined with secretory epithelium, the cells of which were higher and better preserved in the centre of the explant in cultures kept in oxygen and CO2 than in those kept in air. In glands grown in the defined medium in both air and oxygen, irregularly distributed foci of squamous metaplasia appeared in the majority of explants. The metaplasia was either preceded by atrophy, or by hyperplasia and stratification of the secretory lining epithelium, and in many explants both squamous and secretory epithelium were present in the same alveolus. The squamous changes could be completely suppressed by treatment with vitamin A and it is concluded that they are caused by vitamin A deficiency of the tissue cultured in the defined medium which does not contain this vitamin." @default.
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- W2011128547 title "Hypovitaminosis-A in the mouse prostate gland cultured in chemically defined medium" @default.
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- W2011128547 doi "https://doi.org/10.1016/0014-4827(62)90310-5" @default.
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