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• W2011129353 abstract "Abstract. .Before cell cultures can be used as a tool for metabolic and endocrine research, standardized conditions for stationary and growing cells must be established. Our results with chicken embryo fibroblasts and with WI-38 human fibroblasts demonstrate that the metabolic behaviour of these cells differs depending on whether they are in a stationary phase (without serum) or in a growing phase (with serum). Energy during growth is derived almost exclusively from glycolysis. Two additional factors determine the rate of glucose consumption and lactate production: The glucose concentration in the medium and the density of the cells, i.e. the number of cells per surface area. Using a pulse of labelled thymidine it can be shown that after a delay fibroblasts simultaneously go through the first S-phase under the stimulatory influence of serum. Stationary cells behave metabolically as contact-inhibited cells with regard to glucose consumption and lactate production. It appeals that serum triggers off a sequence of metabolic events including increased glycolysis, thymidine incorporation into DNA and growth." @default.
• W2011129353 created "2016-06-24" @default.
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• W2011129353 date "1973-04-01" @default.
• W2011129353 modified "2023-10-15" @default.
• W2011129353 title "Fibroblasts as an Experimental Tool in Metabolic and Hormone Studies." @default.
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• W2011129353 doi "https://doi.org/10.1111/j.1365-2362.1973.tb00337.x" @default.
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