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• W2011145198 endingPage "20" @default.
• W2011145198 startingPage "1" @default.
• W2011145198 abstract "α4βδ GABA(A) receptors (GABARs) have low CNS expression, but their expression is increased by 48h exposure to the neurosteroid THP (3α-OH-5α[β]-pregnan-20-one). THP also increases the efficacy of δ-containing GABARs acutely, where GABA is a partial agonist. Thus, we examined effects of THP (100 nM) and full GABA agonists at α4β2δ (gaboxadol, 10 μM, and β-alanine, 10 μM-1mM), on surface expression of α4β2δ. To this end, we used an α4 construct tagged with a 3XFLAG (F) epitope or measured expression of native α4 and δ. HEK-293 cells or cultured hippocampal neurons were transfected with α4Fβ2δ and treated 24h later with GABA agonists, THP, GABA plus THP or vehicle (0.01% DMSO) for 0.5 h-48 h. Immunocytochemistry was performed under both non-permeabilized and permeabilized conditions to detect surface and intracellular labeling, respectively, using confocal microscopy. The high efficacy agonists and GABA (1 or 10 μM) plus THP increased α4β2δ surface expression up to 3-fold after 48h, an effect first seen by 0.5h. This effect was not dependent upon the polarity of GABAergic current, although expression was increased by KCC2. Intracellular labeling was decreased while functional expression was confirmed by whole cell patch clamp recordings of responses to GABA agonists. GABA plus THP treatment did not alter the rate of receptor removal from the surface membrane, suggesting that THP-induced α4β2δ expression is likely via receptor insertion. Surface expression of α4β2δ was decreased by rottlerin (10 μM), suggesting a role for PKC-δ. These results suggest that trafficking of α4β2δ GABARs is regulated by high efficacy states." @default.
• W2011145198 created "2016-06-24" @default.
• W2011145198 creator A5022863964 @default.
• W2011145198 creator A5051542151 @default.
• W2011145198 creator A5062876714 @default.
• W2011145198 date "2012-06-01" @default.
• W2011145198 modified "2023-09-30" @default.
• W2011145198 title "Regulation of the surface expression of α4β2δ GABAA receptors by high efficacy states" @default.
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• W2011145198 doi "https://doi.org/10.1016/j.brainres.2012.04.047" @default.
• W2011145198 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3371167" @default.
• W2011145198 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22609410" @default.
• W2011145198 hasPublicationYear "2012" @default.
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