FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011149476> ?p ?o ?g. }

• W2011149476 endingPage "619" @default.
• W2011149476 startingPage "613" @default.
• W2011149476 abstract "Abstract The cytosolic glutathione S-transferase from an Aedes albopictus cell line, C6/36, was sensitive to tetranitromethane, phenylglyoxal or pyridoxal phosphate modification, but was unaffected by N -acetylimidazole or diethyl pyrocarbonate. The extent of inactivation of the enzyme by those sensitive reagents was dependent on reagent concentrations but was biphasic in nature and did not follow pseudo-first-order kinetics. Glutathione, ethacrynic acid, S -(hexyl)glutathione, or S -(2,4-di-nitrophenyl)glutathione gave substantial protection of the enzyme from inactivation by these reagents. The modified enzyme showed varying Michaelis constants for glutathione ( K mGSH ) or 1-chloro-2,4-dinitrobenzene ( K mCDNB ) and a smaller catalytic constant ( k cat . These results indicate the involvement of tyrosine, arginine and lysine residues in the reaction mechanism of the mosquito C6/36 cell glutathione S-transferase. From the results of chemical modification and previous pH studies [Chang G.-G., Tsai L.-N., Tang S.-S., and Wang T.-C. (1994) Arch. Biochem. Biophys. 310, 134–143, we propose that tyrosine residue functions as a general base, promoting ionization of the thiol group of enzyme-bound glutathione and resulting in formation of a more reactive nucleophile thiolate that facilitates conjugation. The essential arginine and lysine residues may participate in maintenance of the correct active center structure." @default.
• W2011149476 created "2016-06-24" @default.
• W2011149476 creator A5028590047 @default.
• W2011149476 creator A5040214720 @default.
• W2011149476 creator A5090731915 @default.
• W2011149476 date "1995-05-01" @default.
• W2011149476 modified "2023-09-27" @default.
• W2011149476 title "Chemical modification of glutathione S-transferase from C6/36, an Aedes albopictus cell line" @default.
• W2011149476 cites W1485355897 @default.
• W2011149476 cites W1486158855 @default.
• W2011149476 cites W148657558 @default.
• W2011149476 cites W1490347122 @default.
• W2011149476 cites W1507111189 @default.
• W2011149476 cites W1544393038 @default.
• W2011149476 cites W1547383512 @default.
• W2011149476 cites W1557726566 @default.
• W2011149476 cites W1572376186 @default.
• W2011149476 cites W1591042311 @default.
• W2011149476 cites W1602721680 @default.
• W2011149476 cites W1787242221 @default.
• W2011149476 cites W1850017910 @default.
• W2011149476 cites W1853587765 @default.
• W2011149476 cites W1968445740 @default.
• W2011149476 cites W1969419384 @default.
• W2011149476 cites W1969768949 @default.
• W2011149476 cites W1987773676 @default.
• W2011149476 cites W1990888085 @default.
• W2011149476 cites W1992528871 @default.
• W2011149476 cites W1994529202 @default.
• W2011149476 cites W1999068901 @default.
• W2011149476 cites W2013574038 @default.
• W2011149476 cites W2013788890 @default.
• W2011149476 cites W2020094433 @default.
• W2011149476 cites W2023287483 @default.
• W2011149476 cites W2040535337 @default.
• W2011149476 cites W2049857499 @default.
• W2011149476 cites W2058726456 @default.
• W2011149476 cites W2073859835 @default.
• W2011149476 cites W2075772525 @default.
• W2011149476 cites W2079178286 @default.
• W2011149476 cites W2087930532 @default.
• W2011149476 cites W2154011403 @default.
• W2011149476 cites W2336348371 @default.
• W2011149476 cites W337892304 @default.
• W2011149476 cites W4293247451 @default.
• W2011149476 cites W8040729 @default.
• W2011149476 cites W2086284569 @default.
• W2011149476 doi "https://doi.org/10.1016/0965-1748(94)00101-m" @default.
• W2011149476 hasPublicationYear "1995" @default.
• W2011149476 type Work @default.
• W2011149476 sameAs 2011149476 @default.
• W2011149476 citedByCount "4" @default.
• W2011149476 countsByYear W20111494762021 @default.
• W2011149476 crossrefType "journal-article" @default.
• W2011149476 hasAuthorship W2011149476A5028590047 @default.
• W2011149476 hasAuthorship W2011149476A5040214720 @default.
• W2011149476 hasAuthorship W2011149476A5090731915 @default.
• W2011149476 hasConcept C153911025 @default.
• W2011149476 hasConcept C159047783 @default.
• W2011149476 hasConcept C173758957 @default.
• W2011149476 hasConcept C181199279 @default.
• W2011149476 hasConcept C2776853612 @default.
• W2011149476 hasConcept C2776907368 @default.
• W2011149476 hasConcept C2777775583 @default.
• W2011149476 hasConcept C2780688631 @default.
• W2011149476 hasConcept C533803919 @default.
• W2011149476 hasConcept C538909803 @default.
• W2011149476 hasConcept C55493867 @default.
• W2011149476 hasConcept C59822182 @default.
• W2011149476 hasConcept C86803240 @default.
• W2011149476 hasConceptScore W2011149476C153911025 @default.
• W2011149476 hasConceptScore W2011149476C159047783 @default.
• W2011149476 hasConceptScore W2011149476C173758957 @default.
• W2011149476 hasConceptScore W2011149476C181199279 @default.
• W2011149476 hasConceptScore W2011149476C2776853612 @default.
• W2011149476 hasConceptScore W2011149476C2776907368 @default.
• W2011149476 hasConceptScore W2011149476C2777775583 @default.
• W2011149476 hasConceptScore W2011149476C2780688631 @default.
• W2011149476 hasConceptScore W2011149476C533803919 @default.
• W2011149476 hasConceptScore W2011149476C538909803 @default.
• W2011149476 hasConceptScore W2011149476C55493867 @default.
• W2011149476 hasConceptScore W2011149476C59822182 @default.
• W2011149476 hasConceptScore W2011149476C86803240 @default.
• W2011149476 hasIssue "5" @default.
• W2011149476 hasLocation W20111494761 @default.
• W2011149476 hasOpenAccess W2011149476 @default.
• W2011149476 hasPrimaryLocation W20111494761 @default.
• W2011149476 hasRelatedWork W1850250705 @default.
• W2011149476 hasRelatedWork W1968442673 @default.
• W2011149476 hasRelatedWork W1975114493 @default.
• W2011149476 hasRelatedWork W2053470384 @default.
• W2011149476 hasRelatedWork W217023196 @default.
• W2011149476 hasRelatedWork W2342341740 @default.
• W2011149476 hasRelatedWork W2361536690 @default.
• W2011149476 hasRelatedWork W2374144237 @default.
• W2011149476 hasRelatedWork W2795713980 @default.
• W2011149476 hasRelatedWork W3148426222 @default.
• W2011149476 hasVolume "25" @default.

• next