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• W2011170062 abstract "A capillary gas chromatographic (GC) method using selected-ion monitoring (SIM) was developed for the analysis of cotinine (C.A.S. No. 486-56-6) in human urine. The method is based on basic extraction of cotinine from 2 ml of urine into dichloromethane. After evaporation of the dichloromethane solution to dryness, 100 microliters of toluene were added, prior to GC-mass spectrometric (MS) analysis. Trideuterated cotinine (C.A.S. No. 97664-65-8) was used as the internal standard. More than 1000 automatic chromatographic analyses were made without column degradation. Molecular ions (M) of cotinine and trideuterated cotinine, (m/e = 176 and 179), were monitored in the electron impact (EI) mode and m/e = 177 (M + 1) and m/e = 180 (M + 1) in the chemical ionization (CI) mode with isobutane. The correlation coefficient with SIM and EI was 0.998 (5-20 ng/ml) and with CI was (0.2-2 ng/ml). For thermionic specific detection the correlation coefficient was 0.998 (10-510 ng/ml). Only capillary columns with an apolar bonded stationary phase film thickness of 1 micron showed sufficient inertness for cotinine analysis at the sub ng/ml level. The relative standard deviations for 5 and 20 ng/ml were 5.2 and 3.5% respectively (n = 12) using EI. Spiked urine samples from six non-smokers (5 ng/ml) showed a relative standard deviation of 5%. The overall recovery (25 ng/ml) was 100 +/- 4%. The minimum detectable concentration, using SIM, was ca. 2 ng/ml in the EI mode and ca. 0.2 ng/ml in the CI mode. The half-time for cotinine was ca. 18 h for both active smokers and non-smokers." @default.
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• W2011170062 date "1988-01-01" @default.
• W2011170062 modified "2023-10-16" @default.
• W2011170062 title "Determination of cotinine in urine using glass capillary gas chromatography and selective detection, with special reference to the biological monitoring of passive smoking" @default.
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• W2011170062 doi "https://doi.org/10.1016/s0021-9673(00)88622-7" @default.
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