FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W201119457> ?p ?o ?g. }

• W201119457 endingPage "1207" @default.
• W201119457 startingPage "1201" @default.
• W201119457 abstract "OBJECTIVES The purpose of the study was to investigate the role of nitric oxide (NO) in monocyte-endothelial interaction by augmenting NO release via transfection of human endothelial cells (ECs) with EC NO synthase (eNOS) DNA. BACKGROUND Enhancement of NO synthesis by L-arginine or shear stress reduces endothelial adhesiveness for monocytes and inhibits atherogenesis. To elucidate further the underlying mechanism, we augmented NO synthase expression by transfection of human EC. METHODS Liposome-mediated transfection of EC was performed with a plasmid construct containing the gene encoding eNOS. Expression of eNOS was confirmed by reverse transcription–polymerase chain reaction (RT-PCR). Endothelial cells were exposed to human monocytoid cells, and adherent cells were quantitated using a computer-assisted program. Nitric oxide was measured by chemiluminescence. RESULTS The NO levels were not different in EC that were either not transfected, transfected with beta-gal or liposomes only. The nitric oxide synthase (NOS) transfection increased NO release by +60% (n = 6), which increased further when EC were stimulated by shear stress (24 h) by +137% (n = 5) as compared with untransfected, unstimulated EC (both p < 0.05). The RT-PCR revealed diminished monocyte chemotactic protein-1 (MCP-1) expression in eNOS transfected EC. There was an inverse relation between NO levels and monocyte binding (r = −0.5669, p < 0.002). Stimulation of EC with tumor necrosis factor-alpha (TNF-alpha; 250 U/ml) led to a decrease in NO synthesis, and an increase in monocyte binding. Cells transfected with NOS were resistant to both effects of TNF-alpha. CONCLUSIONS Endothelial cells transfected with eNOS synthesize an increased amount of NO; this is associated with diminished MCP-1 expression and monocyte-endothelial binding. The reduction in monocyte-endothelial binding persists even after cytokine stimulation." @default.
• W201119457 created "2016-06-24" @default.
• W201119457 creator A5010081940 @default.
• W201119457 creator A5039670487 @default.
• W201119457 creator A5058198453 @default.
• W201119457 creator A5067779338 @default.
• W201119457 creator A5082864592 @default.
• W201119457 date "1999-10-01" @default.
• W201119457 modified "2023-09-23" @default.
• W201119457 title "Gene transfer of nitric oxide synthase" @default.
• W201119457 cites W1963781173 @default.
• W201119457 cites W1968141837 @default.
• W201119457 cites W1969703987 @default.
• W201119457 cites W1997640957 @default.
• W201119457 cites W2003677326 @default.
• W201119457 cites W2007499397 @default.
• W201119457 cites W2010715396 @default.
• W201119457 cites W2015789556 @default.
• W201119457 cites W2021934088 @default.
• W201119457 cites W2031131334 @default.
• W201119457 cites W2033974115 @default.
• W201119457 cites W2054949240 @default.
• W201119457 cites W2058344926 @default.
• W201119457 cites W2073861766 @default.
• W201119457 cites W2076995420 @default.
• W201119457 cites W2080515968 @default.
• W201119457 cites W2083285004 @default.
• W201119457 cites W2087576623 @default.
• W201119457 cites W2103079216 @default.
• W201119457 cites W2106478344 @default.
• W201119457 cites W2111787291 @default.
• W201119457 cites W2123706039 @default.
• W201119457 cites W2139533485 @default.
• W201119457 cites W2150775643 @default.
• W201119457 cites W2160301642 @default.
• W201119457 cites W2162010936 @default.
• W201119457 cites W2163628914 @default.
• W201119457 cites W2172296189 @default.
• W201119457 cites W4294216491 @default.
• W201119457 cites W72464374 @default.
• W201119457 doi "https://doi.org/10.1016/s0735-1097(99)00304-6" @default.
• W201119457 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10520813" @default.
• W201119457 hasPublicationYear "1999" @default.
• W201119457 type Work @default.
• W201119457 sameAs 201119457 @default.
• W201119457 citedByCount "45" @default.
• W201119457 countsByYear W2011194572012 @default.
• W201119457 countsByYear W2011194572015 @default.
• W201119457 countsByYear W2011194572016 @default.
• W201119457 countsByYear W2011194572017 @default.
• W201119457 countsByYear W2011194572019 @default.
• W201119457 countsByYear W2011194572020 @default.
• W201119457 countsByYear W2011194572022 @default.
• W201119457 crossrefType "journal-article" @default.
• W201119457 hasAuthorship W201119457A5010081940 @default.
• W201119457 hasAuthorship W201119457A5039670487 @default.
• W201119457 hasAuthorship W201119457A5058198453 @default.
• W201119457 hasAuthorship W201119457A5067779338 @default.
• W201119457 hasAuthorship W201119457A5082864592 @default.
• W201119457 hasBestOaLocation W2011194571 @default.
• W201119457 hasConcept C104317684 @default.
• W201119457 hasConcept C126322002 @default.
• W201119457 hasConcept C153911025 @default.
• W201119457 hasConcept C17991360 @default.
• W201119457 hasConcept C203014093 @default.
• W201119457 hasConcept C2777622882 @default.
• W201119457 hasConcept C2778326061 @default.
• W201119457 hasConcept C2781184567 @default.
• W201119457 hasConcept C519581460 @default.
• W201119457 hasConcept C54009773 @default.
• W201119457 hasConcept C55493867 @default.
• W201119457 hasConcept C71924100 @default.
• W201119457 hasConcept C86803240 @default.
• W201119457 hasConceptScore W201119457C104317684 @default.
• W201119457 hasConceptScore W201119457C126322002 @default.
• W201119457 hasConceptScore W201119457C153911025 @default.
• W201119457 hasConceptScore W201119457C17991360 @default.
• W201119457 hasConceptScore W201119457C203014093 @default.
• W201119457 hasConceptScore W201119457C2777622882 @default.
• W201119457 hasConceptScore W201119457C2778326061 @default.
• W201119457 hasConceptScore W201119457C2781184567 @default.
• W201119457 hasConceptScore W201119457C519581460 @default.
• W201119457 hasConceptScore W201119457C54009773 @default.
• W201119457 hasConceptScore W201119457C55493867 @default.
• W201119457 hasConceptScore W201119457C71924100 @default.
• W201119457 hasConceptScore W201119457C86803240 @default.
• W201119457 hasIssue "4" @default.
• W201119457 hasLocation W2011194571 @default.
• W201119457 hasLocation W2011194572 @default.
• W201119457 hasOpenAccess W201119457 @default.
• W201119457 hasPrimaryLocation W2011194571 @default.
• W201119457 hasRelatedWork W1978327381 @default.
• W201119457 hasRelatedWork W1987269973 @default.
• W201119457 hasRelatedWork W2030742850 @default.
• W201119457 hasRelatedWork W2051267793 @default.
• W201119457 hasRelatedWork W2115648058 @default.
• W201119457 hasRelatedWork W2134382614 @default.
• W201119457 hasRelatedWork W2289257592 @default.

• next