Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011196679> ?p ?o ?g. }
- W2011196679 endingPage "1303" @default.
- W2011196679 startingPage "1294" @default.
- W2011196679 abstract "Although the recently-developed Gemcitabine (GEM) has renewed interest in clinical research in pancreatic carcinoma, it offers modest improvement of tumor-related symptoms and marginal survival advantage, even when combined with other currently-available chemotherapeutic agents such as 5-Fluorouracil (5-FU). We hypothesized that this disappointing result could be due to an interaction between the two drugs affecting cytotoxic activity. We measured in-vitro growth inhibition, cell cycle distribution, gene and protein expression of apoptosis regulators bcl-2, bcl-x and survivin, NFkappaB and telomerase activities of human pancreatic carcinoma cell line Capan-2 following exposure to GEM and 5-FU singly or combined, by MTT assay and median effect analysis, flow cytometry, real-time RT-PCR, Western blotting, electrophoretic mobility shift assay (EMSA) and telomeric repeat amplification protocol (TRAP) assay, respectively. We found cell growth to be inhibited by both drugs, decreasing the percentage of cells in S and G2/M phases and inducing apoptosis, dependent on the levels of bcl-2, bcl-xL and survivin expression in the case of 5-FU, but not for GEM. Moreover, while telomerase activity was reduced equally by both drugs, 5-FU but not GEM effectively downregulated NFkappaB binding activity. Intriguingly, a substantial antagonistic effect was noticed when GEM was combined with 5-FU in the concentration range tested, with the exception of the TRAP assay. These indications of an antagonistic interaction between GEM and 5-FU in some pancreatic cancer context urge further investigation of both genetic and non-genetic differences to identify the variables most relevant for optimal selection and dosing of treatment for the individual patient." @default.
- W2011196679 created "2016-06-24" @default.
- W2011196679 creator A5001434615 @default.
- W2011196679 creator A5005189551 @default.
- W2011196679 creator A5009399985 @default.
- W2011196679 creator A5020736353 @default.
- W2011196679 creator A5025326342 @default.
- W2011196679 creator A5029193409 @default.
- W2011196679 creator A5030327214 @default.
- W2011196679 creator A5046643641 @default.
- W2011196679 creator A5047980009 @default.
- W2011196679 creator A5048855892 @default.
- W2011196679 date "2006-10-11" @default.
- W2011196679 modified "2023-09-28" @default.
- W2011196679 title "Antagonistic interactions between gemcitabine and 5-fluorouracil in the human pancreatic carcinoma cell line capan-2" @default.
- W2011196679 cites W1523461418 @default.
- W2011196679 cites W1542523185 @default.
- W2011196679 cites W1595815978 @default.
- W2011196679 cites W1768187423 @default.
- W2011196679 cites W1931508686 @default.
- W2011196679 cites W1961680179 @default.
- W2011196679 cites W1963862070 @default.
- W2011196679 cites W1978237191 @default.
- W2011196679 cites W1980253503 @default.
- W2011196679 cites W1982551629 @default.
- W2011196679 cites W1982885349 @default.
- W2011196679 cites W1985068997 @default.
- W2011196679 cites W1987768599 @default.
- W2011196679 cites W1988036144 @default.
- W2011196679 cites W1991865867 @default.
- W2011196679 cites W1992960292 @default.
- W2011196679 cites W1994017228 @default.
- W2011196679 cites W1994410190 @default.
- W2011196679 cites W1995215542 @default.
- W2011196679 cites W1997208169 @default.
- W2011196679 cites W1999605095 @default.
- W2011196679 cites W2000932071 @default.
- W2011196679 cites W2003618771 @default.
- W2011196679 cites W2008600959 @default.
- W2011196679 cites W2009599943 @default.
- W2011196679 cites W2009970556 @default.
- W2011196679 cites W2010831478 @default.
- W2011196679 cites W2010833449 @default.
- W2011196679 cites W2012076841 @default.
- W2011196679 cites W2013694684 @default.
- W2011196679 cites W2014742104 @default.
- W2011196679 cites W2018306978 @default.
- W2011196679 cites W2019252036 @default.
- W2011196679 cites W2020485312 @default.
- W2011196679 cites W2024210946 @default.
- W2011196679 cites W2027943817 @default.
- W2011196679 cites W2028382049 @default.
- W2011196679 cites W2029324582 @default.
- W2011196679 cites W2039791984 @default.
- W2011196679 cites W2042502745 @default.
- W2011196679 cites W2045025607 @default.
- W2011196679 cites W2045114648 @default.
- W2011196679 cites W2045324074 @default.
- W2011196679 cites W2053170633 @default.
- W2011196679 cites W2058115449 @default.
- W2011196679 cites W2062513246 @default.
- W2011196679 cites W2063176993 @default.
- W2011196679 cites W2065392301 @default.
- W2011196679 cites W2071968624 @default.
- W2011196679 cites W2077945404 @default.
- W2011196679 cites W2082777374 @default.
- W2011196679 cites W2083723846 @default.
- W2011196679 cites W2085419794 @default.
- W2011196679 cites W2091030447 @default.
- W2011196679 cites W2094646101 @default.
- W2011196679 cites W2098437774 @default.
- W2011196679 cites W2099249565 @default.
- W2011196679 cites W2099273077 @default.
- W2011196679 cites W2103607678 @default.
- W2011196679 cites W2103629572 @default.
- W2011196679 cites W2123344367 @default.
- W2011196679 cites W2124261230 @default.
- W2011196679 cites W2134129734 @default.
- W2011196679 cites W2136664234 @default.
- W2011196679 cites W2149548143 @default.
- W2011196679 cites W2150435224 @default.
- W2011196679 cites W2166994863 @default.
- W2011196679 cites W2167490100 @default.
- W2011196679 cites W222088725 @default.
- W2011196679 cites W234950505 @default.
- W2011196679 cites W2400630459 @default.
- W2011196679 cites W2402827688 @default.
- W2011196679 cites W2412643659 @default.
- W2011196679 cites W2414261930 @default.
- W2011196679 cites W2461357508 @default.
- W2011196679 cites W3134530972 @default.
- W2011196679 cites W58971687 @default.
- W2011196679 cites W2414257022 @default.
- W2011196679 doi "https://doi.org/10.4161/cbt.5.10.3152" @default.
- W2011196679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16929163" @default.
- W2011196679 hasPublicationYear "2006" @default.
- W2011196679 type Work @default.
- W2011196679 sameAs 2011196679 @default.