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• W2011210588 abstract "The concentration of photochemical reaction centers in spinach chloroplasts was measured with direct spectrophotometric techniques. Quantitation of Photosystem II (PS II) was implemented (a) from the light-induced absorbance change in the near-ultraviolet (ΔA320), monitoring the primary quinone acceptor QA of PS II; and (b) from the light-induced absorbance change in the green region (ΔA540–550), monitoring the band-shift C550 of the PS II reaction center pheophytin molecule. Both measurements yielded identical chlorophyll (Chl) per PS II ratios (ChlPS II = 380:1). Hydroxylamine treatment and the loss of Mn from the oxygen-evolving enzyme of photosynthesis had no effect on the quantitation of QA by ΔA320. It is concluded that, under the experimental conditions employed, measurements of QA photoreduction are not overlapped by state transitions of the water-splitting enzyme. Quantitation of Photosystem I (PS I) was implemented from the light-induced absorbance change in the red region of the spectrum (ΔA700), monitoring photooxidation of the PS I reaction center P-700. A ChlPS I = 650:1 was derived. Thus, a PS IIPS I stoichiometric ratio of 1.7 was estimated in spinach chloroplasts." @default.
• W2011210588 created "2016-06-24" @default.
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• W2011210588 date "1986-04-01" @default.
• W2011210588 modified "2023-09-26" @default.
• W2011210588 title "Quantitation of photosystem II in spinach chloroplasts" @default.
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• W2011210588 doi "https://doi.org/10.1016/0005-2728(86)90023-x" @default.
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