FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011241659> ?p ?o ?g. }

• W2011241659 endingPage "254" @default.
• W2011241659 startingPage "243" @default.
• W2011241659 abstract "Tarocystatin (CeCPI) from taro (Colocasia esculenta cv. Kaohsiung no. 1), a group-2 phytocystatin, shares a conserved N-terminal cystatin domain (NtD) with other phytocystatins but contains a C-terminal cystatin-like extension (CtE). The structure of the tarocystatin-papain complex and the domain interaction between NtD and CtE in tarocystatin have not been determined. We resolved the crystal structure of the phytocystatin-papain complex at resolution 2.03 Å. Surprisingly, the structure of the NtD-papain complex in a stoichiometry of 1:1 could be built, with no CtE observed. Only two remnant residues of CtE could be built in the structure of the CtE-papain complex. Therefore, CtE is easily digested by papain. To further characterize the interaction between NtD and CtE, three segments of tarocystatin, including the full-length (FL), NtD and CtE, were used to analyze the domain-domain interaction and the inhibition ability. The results from glutaraldehyde cross-linking and yeast two-hybrid assay indicated the existence of an intrinsic flexibility in the region linking NtD and CtE for most tarocystatin molecules. In the inhibition activity assay, the glutathione-S-transferase (GST)-fused FL showed the highest inhibition ability without residual peptidase activity, and GST-NtD and FL showed almost the same inhibition ability, which was higher than with NtD alone. On the basis of the structures, the linker flexibility and inhibition activity of tarocystatins, we propose that the overhangs from the cystatin domain may enhance the inhibition ability of the cystatin domain against papain." @default.
• W2011241659 created "2016-06-24" @default.
• W2011241659 creator A5021502041 @default.
• W2011241659 creator A5037524264 @default.
• W2011241659 creator A5057027788 @default.
• W2011241659 creator A5083528311 @default.
• W2011241659 creator A5091505117 @default.
• W2011241659 date "2011-03-18" @default.
• W2011241659 modified "2023-10-06" @default.
• W2011241659 title "Crystal structure of tarocystatin–papain complex: implications for the inhibition property of group-2 phytocystatins" @default.
• W2011241659 cites W1493623063 @default.
• W2011241659 cites W1521251288 @default.
• W2011241659 cites W1539796472 @default.
• W2011241659 cites W1561708153 @default.
• W2011241659 cites W1965015418 @default.
• W2011241659 cites W1965702707 @default.
• W2011241659 cites W1975607257 @default.
• W2011241659 cites W1978322999 @default.
• W2011241659 cites W1984163556 @default.
• W2011241659 cites W1995017064 @default.
• W2011241659 cites W1997985638 @default.
• W2011241659 cites W2001752402 @default.
• W2011241659 cites W2012439680 @default.
• W2011241659 cites W2014694459 @default.
• W2011241659 cites W2021285425 @default.
• W2011241659 cites W2024297705 @default.
• W2011241659 cites W2033262167 @default.
• W2011241659 cites W2035503835 @default.
• W2011241659 cites W2041905742 @default.
• W2011241659 cites W2045988593 @default.
• W2011241659 cites W2059071850 @default.
• W2011241659 cites W2068917459 @default.
• W2011241659 cites W2073183586 @default.
• W2011241659 cites W2077595384 @default.
• W2011241659 cites W2082798390 @default.
• W2011241659 cites W2084262408 @default.
• W2011241659 cites W2085663169 @default.
• W2011241659 cites W2086924990 @default.
• W2011241659 cites W2090586240 @default.
• W2011241659 cites W2092426259 @default.
• W2011241659 cites W2095605951 @default.
• W2011241659 cites W2098443736 @default.
• W2011241659 cites W2103776223 @default.
• W2011241659 cites W2104246038 @default.
• W2011241659 cites W2108490599 @default.
• W2011241659 cites W2123983212 @default.
• W2011241659 cites W2131941267 @default.
• W2011241659 cites W2132670203 @default.
• W2011241659 cites W2138460330 @default.
• W2011241659 cites W2144081223 @default.
• W2011241659 cites W2150595596 @default.
• W2011241659 cites W2152811683 @default.
• W2011241659 cites W2157558988 @default.
• W2011241659 cites W2158783623 @default.
• W2011241659 cites W4210323379 @default.
• W2011241659 doi "https://doi.org/10.1007/s00425-011-1398-8" @default.
• W2011241659 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3144364" @default.
• W2011241659 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21416241" @default.
• W2011241659 hasPublicationYear "2011" @default.
• W2011241659 type Work @default.
• W2011241659 sameAs 2011241659 @default.
• W2011241659 citedByCount "36" @default.
• W2011241659 countsByYear W20112416592012 @default.
• W2011241659 countsByYear W20112416592013 @default.
• W2011241659 countsByYear W20112416592014 @default.
• W2011241659 countsByYear W20112416592015 @default.
• W2011241659 countsByYear W20112416592016 @default.
• W2011241659 countsByYear W20112416592017 @default.
• W2011241659 countsByYear W20112416592018 @default.
• W2011241659 countsByYear W20112416592019 @default.
• W2011241659 countsByYear W20112416592020 @default.
• W2011241659 countsByYear W20112416592021 @default.
• W2011241659 countsByYear W20112416592022 @default.
• W2011241659 countsByYear W20112416592023 @default.
• W2011241659 crossrefType "journal-article" @default.
• W2011241659 hasAuthorship W2011241659A5021502041 @default.
• W2011241659 hasAuthorship W2011241659A5037524264 @default.
• W2011241659 hasAuthorship W2011241659A5057027788 @default.
• W2011241659 hasAuthorship W2011241659A5083528311 @default.
• W2011241659 hasAuthorship W2011241659A5091505117 @default.
• W2011241659 hasBestOaLocation W20112416591 @default.
• W2011241659 hasConcept C111919701 @default.
• W2011241659 hasConcept C12554922 @default.
• W2011241659 hasConcept C159641895 @default.
• W2011241659 hasConcept C181199279 @default.
• W2011241659 hasConcept C185592680 @default.
• W2011241659 hasConcept C2779201268 @default.
• W2011241659 hasConcept C2779548226 @default.
• W2011241659 hasConcept C2779832904 @default.
• W2011241659 hasConcept C2780412452 @default.
• W2011241659 hasConcept C2780557392 @default.
• W2011241659 hasConcept C41008148 @default.
• W2011241659 hasConcept C55493867 @default.
• W2011241659 hasConcept C71240020 @default.
• W2011241659 hasConcept C86803240 @default.
• W2011241659 hasConceptScore W2011241659C111919701 @default.
• W2011241659 hasConceptScore W2011241659C12554922 @default.
• W2011241659 hasConceptScore W2011241659C159641895 @default.

• next