Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011252886> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2011252886 endingPage "7632" @default.
- W2011252886 startingPage "7625" @default.
- W2011252886 abstract "The insect cell/baculovirus system was used to express the mercurial-insensitive water channel aquaporin-4 (AQP4) for purification and reconstitution. Immunoblot analysis of Sf9 cells infected with recombinant baculovirus showed greatest AQP4 expression at 72 h after infection at a multiplicity-of-infection of 5. Immunostaining and cell membrane fractionation indicated AQP4 plasma membrane expression. Quantitative immunoblot analysis showed ∼60 μg of AQP4 per milligram of plasma membrane protein (∼2 mg of AQP4 protein per liter of Sf9 cell culture). Functional analysis by stopped-flow light scattering indicated that AQP4 functioned as a mercurial-insensitive water-selective transporter. Osmotic water permeability (Pf) in plasma membrane vesicles from AQP4-expressing Sf9 cells was very high (0.053 cm/s at 10 °C), weakly temperature dependent (activation energy, 4.5 kcal/mol), and not inhibited by HgCl2. The AQP4 single channel water permeability (pf), estimated from Pf and protein amount, was 19 × 10-14 cm3/s. Purification of AQP4 to a single Coomassie blue-stained protein on SDS−PAGE (1300-fold over homogenate) was achieved by membrane fractionation, carbonate stripping of nonintegral proteins, solubilization in octyl-β-glucoside, and anion exchange chromatography. AQP4 protein identity was confirmed by mass spectrometry. Reconstitution of purified AQP4 into proteoliposomes increased osmotic water permeability by >40-fold, giving a pf of 15 × 10-14 cm3/s, remarkably greater than that of 4.9 × 10-14 cm3/s measured in parallel for AQP1. These results establish the first purification of an aquaporin from a heterologous expression system. The high AQP4 pf suggests (a) significant functional differences among the aquaporins, (b) inadequacy of existing pore models to account for high water flow and water permselectivity, and (c) possible enhancement of water flow by AQP4 assembly in orthogonal arrays." @default.
- W2011252886 created "2016-06-24" @default.
- W2011252886 creator A5047160585 @default.
- W2011252886 creator A5072978671 @default.
- W2011252886 creator A5087849281 @default.
- W2011252886 date "1997-06-01" @default.
- W2011252886 modified "2023-09-30" @default.
- W2011252886 title "Very High Single Channel Water Permeability of Aquaporin-4 in Baculovirus-Infected Insect Cells and Liposomes Reconstituted with Purified Aquaporin-4" @default.
- W2011252886 cites W1504938832 @default.
- W2011252886 cites W1509371781 @default.
- W2011252886 cites W1537465580 @default.
- W2011252886 cites W1607563537 @default.
- W2011252886 cites W1944111067 @default.
- W2011252886 cites W1981799488 @default.
- W2011252886 cites W1989947773 @default.
- W2011252886 cites W1998770029 @default.
- W2011252886 cites W2010132581 @default.
- W2011252886 cites W2035303681 @default.
- W2011252886 cites W2051483207 @default.
- W2011252886 cites W2062716068 @default.
- W2011252886 cites W2071375439 @default.
- W2011252886 cites W2083070757 @default.
- W2011252886 cites W2118555110 @default.
- W2011252886 cites W2158459926 @default.
- W2011252886 cites W2170463631 @default.
- W2011252886 doi "https://doi.org/10.1021/bi970231r" @default.
- W2011252886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9200715" @default.
- W2011252886 hasPublicationYear "1997" @default.
- W2011252886 type Work @default.
- W2011252886 sameAs 2011252886 @default.
- W2011252886 citedByCount "104" @default.
- W2011252886 countsByYear W20112528862012 @default.
- W2011252886 countsByYear W20112528862013 @default.
- W2011252886 countsByYear W20112528862014 @default.
- W2011252886 countsByYear W20112528862015 @default.
- W2011252886 countsByYear W20112528862016 @default.
- W2011252886 countsByYear W20112528862017 @default.
- W2011252886 countsByYear W20112528862018 @default.
- W2011252886 countsByYear W20112528862019 @default.
- W2011252886 countsByYear W20112528862020 @default.
- W2011252886 countsByYear W20112528862021 @default.
- W2011252886 countsByYear W20112528862022 @default.
- W2011252886 countsByYear W20112528862023 @default.
- W2011252886 crossrefType "journal-article" @default.
- W2011252886 hasAuthorship W2011252886A5047160585 @default.
- W2011252886 hasAuthorship W2011252886A5072978671 @default.
- W2011252886 hasAuthorship W2011252886A5087849281 @default.
- W2011252886 hasConcept C103878061 @default.
- W2011252886 hasConcept C104317684 @default.
- W2011252886 hasConcept C12554922 @default.
- W2011252886 hasConcept C153911025 @default.
- W2011252886 hasConcept C154280991 @default.
- W2011252886 hasConcept C15755913 @default.
- W2011252886 hasConcept C185592680 @default.
- W2011252886 hasConcept C2777773057 @default.
- W2011252886 hasConcept C40767141 @default.
- W2011252886 hasConcept C43617362 @default.
- W2011252886 hasConcept C55493867 @default.
- W2011252886 hasConcept C86803240 @default.
- W2011252886 hasConcept C97428945 @default.
- W2011252886 hasConceptScore W2011252886C103878061 @default.
- W2011252886 hasConceptScore W2011252886C104317684 @default.
- W2011252886 hasConceptScore W2011252886C12554922 @default.
- W2011252886 hasConceptScore W2011252886C153911025 @default.
- W2011252886 hasConceptScore W2011252886C154280991 @default.
- W2011252886 hasConceptScore W2011252886C15755913 @default.
- W2011252886 hasConceptScore W2011252886C185592680 @default.
- W2011252886 hasConceptScore W2011252886C2777773057 @default.
- W2011252886 hasConceptScore W2011252886C40767141 @default.
- W2011252886 hasConceptScore W2011252886C43617362 @default.
- W2011252886 hasConceptScore W2011252886C55493867 @default.
- W2011252886 hasConceptScore W2011252886C86803240 @default.
- W2011252886 hasConceptScore W2011252886C97428945 @default.
- W2011252886 hasIssue "24" @default.
- W2011252886 hasLocation W20112528861 @default.
- W2011252886 hasLocation W20112528862 @default.
- W2011252886 hasOpenAccess W2011252886 @default.
- W2011252886 hasPrimaryLocation W20112528861 @default.
- W2011252886 hasRelatedWork W1496743899 @default.
- W2011252886 hasRelatedWork W1974554319 @default.
- W2011252886 hasRelatedWork W1974732373 @default.
- W2011252886 hasRelatedWork W2011252886 @default.
- W2011252886 hasRelatedWork W2079156087 @default.
- W2011252886 hasRelatedWork W2345890204 @default.
- W2011252886 hasRelatedWork W2355977992 @default.
- W2011252886 hasRelatedWork W2413740037 @default.
- W2011252886 hasRelatedWork W4319773713 @default.
- W2011252886 hasRelatedWork W4385186047 @default.
- W2011252886 hasVolume "36" @default.
- W2011252886 isParatext "false" @default.
- W2011252886 isRetracted "false" @default.
- W2011252886 magId "2011252886" @default.
- W2011252886 workType "article" @default.