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- W2011269747 abstract "Transient Receptor Potential Vallinoid type 1 (TRPV1) channel is a voltage-, heat-, and ligand-activated, nonselective cation channel. As a member of hexahelical cation channel superfamily, TRPV1 does not feature the positively charged S4, which is responsible for the voltage sensitivity of voltage-gated ion channels (VGICs). The origin for TRPV1's voltage sensitivity must lie elsewhere. The objective of this study was to investigate the role of “classical” S1-S4 voltage sensor, in particular S4, in the TRPV1, and obtain molecular information about the functional coupling between voltage, chemical and temperature sensors. To test whether the positive charge is involved in activation and to explore the function of the S4 segment in TRPV1 activation, we made a series of single point arginine replacements that scanned residues from E536 through T556 in the S4 and characterized their response to capsaicin, protons and heat using cut-open oocyte voltage-clamp. We also constructed TRPV1/Shaker chimeras in which the S1-S4, S3-S4, or S4 segments of the TRPV1 channel were replaced by the corresponding segments of Shaker channels. The introduction of positively charged residues in the S4 segment resulted in a steeper activation curve and a defective response to capsaicin and protons. Importantly, several of the mutant channels displayed strong inhibition of hyperpolarization-activated inward currents. Three chimeras gave rise to functional channels that exhibited stronger voltage dependence at positive voltages than TRPV1. The chimeras progressively lost inward current with increasing portions replaced by the corresponding Shaker region. They were activated by protons with higher sensitivity compared with TRPV1. These results suggest that re-established outward rectification was dominant over other gating stimuli; in other words, keeping the S1-S4 in the “deactivated” state prevented activation by protons, capsaicin or temperature. In wildtype TRPV1, the S4 seems to inhibit activation by protons." @default.
- W2011269747 created "2016-06-24" @default.
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- W2011269747 date "2015-01-01" @default.
- W2011269747 modified "2023-10-02" @default.
- W2011269747 title "Role of the Voltage Sensing Domain S1-S4 in TRPV1 Channels" @default.
- W2011269747 doi "https://doi.org/10.1016/j.bpj.2014.11.2337" @default.
- W2011269747 hasPublicationYear "2015" @default.
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