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- W2011276272 abstract "Carvacrol and mild heat treatment were tested for their efficiency to increase the radiosensitivity of Bacillus cereus in broth. The bacterium was treated with γ-irradiation alone or in combination with carvacrol at its minimal inhibitory concentration or mild heat treatment for 10 min at 45 °C. The effects of this combination of treatments were studied on various parameters: the bacterial viability, the modifications of the cell morphology with scanning electron microscopy (SEM), the cellular fatty acids composition of the membrane quantified by gas chromatography, the intracellular and extracellular adenosine 5′-triphosphate (ATP) concentrations, and the DNA degradation. Combined treatments resulted in additive or synergistic effects as compared to γ-irradiation alone. A significant modification (P ≤ 0.05) of the fatty acid composition and unsaturation ratios was observed. Pretreatment with mild heat or carvacrol before irradiation disturbed the membrane integrity of B. cereus and induced a significant decrease (P ≤ 0.05) of the intracellular ATP concentration. SEM observations revealed that the cell membrane was more severely affected with combined treatment than irradiation alone. The electrophoresis analysis showed that DNA degradation by combined treatments was greater than the γ-irradiation alone." @default.
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- W2011276272 date "2010-06-22" @default.
- W2011276272 modified "2023-10-16" @default.
- W2011276272 title "Response of <i>Bacillus cereus</i> to γ-Irradiation in Combination with Carvacrol or Mild Heat Treatment" @default.
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- W2011276272 doi "https://doi.org/10.1021/jf101044f" @default.
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