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- W2011280900 abstract "High spatial resolution and large area thickness mapping of label-free protein microarray has been achieved using imaging ellipsometry (IE) under optimized conditions. The protein patterns with feature size down to 8×8 μm(2) was readily imaged, and the binding between the surface immobilized antigen and the antibody was monitored. Quantitative thickness analysis of antibody-antigen binding on the 32×32 μm(2) micron spots was successfully performed, and we have obtained a limit of detection as low as 1.2 pg/spot. This work demonstrates that appropriately optimized IE could be used as a highly sensitive and high through-put label-free technique for studying surface antigen-antibody recognition in sub-40 μm scale." @default.
- W2011280900 created "2016-06-24" @default.
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- W2011280900 date "2011-08-01" @default.
- W2011280900 modified "2023-10-14" @default.
- W2011280900 title "High spatial resolution label-free detection of antigen–antibody binding on patterned surface by imaging ellipsometry" @default.
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- W2011280900 doi "https://doi.org/10.1016/j.jcis.2011.04.107" @default.
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