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- W2011282261 abstract "The cloning and expression of theHaemophilus influenzaegene,nanA, for the putativeN-acetylneuraminate lyase enzyme, also known asN-acetylneuraminic acid aldolase or sialic acid aldolase, are reported. The gene was isolated from ATCC type strain 49247 and cloned into theEscherichia coliexpression vector pKKtac, which contained the strongtacpromoter. Gene expression was compared with the homologousE. coli nplgene coding for the lyase. Purification protocols for the products of thenanA andnplgenes are presented. Activity analysis showed that thenanA gene product is a sialic acid aldolase with more than threefold greater specific activity (6.9 IU/mg) than the enzyme fromE. coli(≤2 IU/mg). A method for the provision of lyase orthorhombic crystals is reported. These crystals diffract to better than 2.0 Å, which paves the way to the solution of the enzyme's three-dimensional structure." @default.
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- W2011282261 date "1998-04-01" @default.
- W2011282261 modified "2023-10-17" @default.
- W2011282261 title "Expression inEscherichia coliof the PutativeN-Acetylneuraminate Lyase Gene (nanA) fromHaemophilus influenzae:Overproduction, Purification, and Crystallization" @default.
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- W2011282261 doi "https://doi.org/10.1006/prep.1997.0841" @default.
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