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- W2011285864 abstract "Titration of microorganisms in infectious or environmental samples is a corner stone of quantitative microbiology. A simple method is presented to estimate the microbial counts obtained with the serial dilution technique for microorganisms that can grow on bacteriological media and develop into a colony. The number (concentration) of viable microbial organisms is estimated from a single dilution plate (assay) without a need for replicate plates. Our method selects the best agar plate with which to estimate the microbial counts, and takes into account the colony size and plate area that both contribute to the likelihood of miscounting the number of colonies on a plate. The estimate of the optimal count given by our method can be used to narrow the search for the best (optimal) dilution plate and saves time. The required inputs are the plate size, the microbial colony size, and the serial dilution factors. The proposed approach shows relative accuracy well within ± 0.1 log10 from data produced by computer simulations. The method maintains this accuracy even in the presence of dilution errors of up to 10% (for both the aliquot and diluent volumes), microbial counts between 104 and 1012 colony-forming units, dilution ratios from 2 to 100, and plate size to colony size ratios between 6.25 to 200." @default.
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- W2011285864 date "1877-01-01" @default.
- W2011285864 modified "2023-10-16" @default.
- W2011285864 title "Saccharometer or polariscope of M. Laurent" @default.
- W2011285864 doi "https://doi.org/10.1016/0016-0032(77)90171-5" @default.
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