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- W2011308240 abstract "The effect of oxidized dithiothreitol (DTT) on the conformation and function of arginine kinase from shrimp Feneropenaeus chinensis was investigated with the methods of intrinsic fluorescence, ANS fluorescence, size exclusion chromatography (SEC), sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS–PAGE), and activity assay. The excess molecular oxidized dithiothreitol could result in a loss of activity and conformational change of arginine kinase. The oxidized arginine kinase was characterized by monitoring the changes of fluorescence emission wavelength (excitation wavelength: 295 nm) and the intensity of 1-anilino-8-naphthalenesulfonate (ANS) binding (excitation wavelength: 380 nm) to the protein. The results of fluorescence spectra showed that the presence of oxidized DTT could result in a marked change in the enzyme tertiary structure. The conformational changes of native and oxidized arginine kinase are induced by the presence of the full set of transition state analog (TSA) components. The results of size exclusion chromatography and SDS–PAGE indicated that no disulfide bond was formed among the protein molecules in the oxidized-DTT solution.Key words: arginine kinase, oxidized dithiothreitol, conformational change, inactivation." @default.
- W2011308240 created "2016-06-24" @default.
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- W2011308240 date "2004-06-01" @default.
- W2011308240 modified "2023-10-09" @default.
- W2011308240 title "Conformational change and inactivation of arginine kinase from shrimp<i>Feneropenaeus chinensis</i>in oxidized dithiothreitol solutions" @default.
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- W2011308240 doi "https://doi.org/10.1139/o04-033" @default.
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