Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011342651> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2011342651 endingPage "847" @default.
- W2011342651 startingPage "840" @default.
- W2011342651 abstract "Lipases from several strains of the imperfect fungus Geotrichum candidum are renowned for their high, though not absolute, specificity for Δ9-unsaturated fatty acids. Starting from a commercially available raw material (GC-4 lipase from Amano), an expeditious two-step isolation method was developed that allowed us to prepare 260 mg of pure enzyme within 2 days. The 61.6-kDa lipase was homogeneous in SDS-PAGE, had a carbohydrate content of 8.8 wt%, and revealed a microheterogeneic pattern between pH 4.4 and 4.8 on isoelectric focusing. Deglycosylation with endoglycosidase H abolished this microheterogeneity, and the lipase focused as a single band at pH 4.63. Over a broad pH range the deglycosylated enzyme had higher specific activities than the native glycosylated form. In hydrolysis as well as in transesterification, a high preference for oleic acid over saturated fatty acids was found for the GC-4 lipase. Crystals were obtained from the native and deglycosylated enzyme as well as from the lipase modified by p-chloromercuribenzoate and iodine. High-quality crystals of native GC-4 lipase grew from media containing polyethylene glycol 20,000. They diffracted to at least 2.5 Å, the lattice constants were a = 53.1 Å, b = 83.5 Å, c = 57.8 Å, β = 100° and the space group was monoclinic P 21. A space-filling coefficient of 2.3 Å3 was calculated." @default.
- W2011342651 created "2016-06-24" @default.
- W2011342651 creator A5050419271 @default.
- W2011342651 creator A5052911831 @default.
- W2011342651 creator A5058185208 @default.
- W2011342651 creator A5062512066 @default.
- W2011342651 creator A5073187609 @default.
- W2011342651 date "1991-10-01" @default.
- W2011342651 modified "2023-10-16" @default.
- W2011342651 title "Large-scale purification, enzymic characterization, and crystallization of the lipase from Geotrichum candidum" @default.
- W2011342651 cites W1506277265 @default.
- W2011342651 cites W1508048429 @default.
- W2011342651 cites W1585178517 @default.
- W2011342651 cites W1854079265 @default.
- W2011342651 cites W1955605693 @default.
- W2011342651 cites W1966923595 @default.
- W2011342651 cites W1966999658 @default.
- W2011342651 cites W1972600071 @default.
- W2011342651 cites W1977910515 @default.
- W2011342651 cites W1992140336 @default.
- W2011342651 cites W2009570597 @default.
- W2011342651 cites W2012176370 @default.
- W2011342651 cites W2023513562 @default.
- W2011342651 cites W2076045985 @default.
- W2011342651 cites W2100837269 @default.
- W2011342651 cites W2120452919 @default.
- W2011342651 cites W2132178116 @default.
- W2011342651 cites W2143708490 @default.
- W2011342651 cites W2158062270 @default.
- W2011342651 cites W4231876448 @default.
- W2011342651 cites W983619299 @default.
- W2011342651 doi "https://doi.org/10.1016/0141-0229(91)90069-m" @default.
- W2011342651 hasPublicationYear "1991" @default.
- W2011342651 type Work @default.
- W2011342651 sameAs 2011342651 @default.
- W2011342651 citedByCount "22" @default.
- W2011342651 countsByYear W20113426512015 @default.
- W2011342651 countsByYear W20113426512017 @default.
- W2011342651 crossrefType "journal-article" @default.
- W2011342651 hasAuthorship W2011342651A5050419271 @default.
- W2011342651 hasAuthorship W2011342651A5052911831 @default.
- W2011342651 hasAuthorship W2011342651A5058185208 @default.
- W2011342651 hasAuthorship W2011342651A5062512066 @default.
- W2011342651 hasAuthorship W2011342651A5073187609 @default.
- W2011342651 hasConcept C178790620 @default.
- W2011342651 hasConcept C181199279 @default.
- W2011342651 hasConcept C185592680 @default.
- W2011342651 hasConcept C2776406908 @default.
- W2011342651 hasConcept C2777241282 @default.
- W2011342651 hasConcept C2779607525 @default.
- W2011342651 hasConcept C2779697368 @default.
- W2011342651 hasConcept C2780786110 @default.
- W2011342651 hasConcept C2781403372 @default.
- W2011342651 hasConcept C31903555 @default.
- W2011342651 hasConcept C43617362 @default.
- W2011342651 hasConcept C55493867 @default.
- W2011342651 hasConcept C94412978 @default.
- W2011342651 hasConceptScore W2011342651C178790620 @default.
- W2011342651 hasConceptScore W2011342651C181199279 @default.
- W2011342651 hasConceptScore W2011342651C185592680 @default.
- W2011342651 hasConceptScore W2011342651C2776406908 @default.
- W2011342651 hasConceptScore W2011342651C2777241282 @default.
- W2011342651 hasConceptScore W2011342651C2779607525 @default.
- W2011342651 hasConceptScore W2011342651C2779697368 @default.
- W2011342651 hasConceptScore W2011342651C2780786110 @default.
- W2011342651 hasConceptScore W2011342651C2781403372 @default.
- W2011342651 hasConceptScore W2011342651C31903555 @default.
- W2011342651 hasConceptScore W2011342651C43617362 @default.
- W2011342651 hasConceptScore W2011342651C55493867 @default.
- W2011342651 hasConceptScore W2011342651C94412978 @default.
- W2011342651 hasIssue "10" @default.
- W2011342651 hasLocation W20113426511 @default.
- W2011342651 hasOpenAccess W2011342651 @default.
- W2011342651 hasPrimaryLocation W20113426511 @default.
- W2011342651 hasRelatedWork W1966923595 @default.
- W2011342651 hasRelatedWork W1977465195 @default.
- W2011342651 hasRelatedWork W1989325440 @default.
- W2011342651 hasRelatedWork W1994706479 @default.
- W2011342651 hasRelatedWork W2011462175 @default.
- W2011342651 hasRelatedWork W2024577019 @default.
- W2011342651 hasRelatedWork W2084238080 @default.
- W2011342651 hasRelatedWork W2095571065 @default.
- W2011342651 hasRelatedWork W2100056564 @default.
- W2011342651 hasRelatedWork W2381088179 @default.
- W2011342651 hasVolume "13" @default.
- W2011342651 isParatext "false" @default.
- W2011342651 isRetracted "false" @default.
- W2011342651 magId "2011342651" @default.
- W2011342651 workType "article" @default.