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- W2011346118 endingPage "C60" @default.
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- W2011346118 abstract "Pathophysiological conditions challenge cell volume homeostasis and perturb cell volume regulatory mechanisms leading to alterations of cell metabolism, active transepithelial transport, cell migration, and death. We report that inhibition of the 5-lipoxygenase (5-LO) with AA861 or ETH 615-139, the cysteinyl leukotriene 1 receptor (CysLT 1 ) with the antiasthmatic drug Zafirlukast, or the volume-sensitive organic anion channel (VSOAC) with DIDS blocks the release of organic osmolytes (taurine, meAIB) and the concomitant cell volume restoration following hypoosmotic swelling of human type II-like lung epithelial cells (A549). Reactive oxygen species (ROS) are produced in A549 cells upon hypotonic cell swelling by a diphenylene iodonium-sensitive NADPH oxidase. The swelling-induced taurine release is suppressed by ROS scavenging (butylated hydroxytoluene, N-acetyl cysteine) and potentiated by H 2 O 2 . Ca 2+ mobilization with ionomycin or ATP stimulates the swelling-induced taurine release whereas calmodulin inhibition (W7) inhibits the release. Chelation of the extracellular Ca 2+ (EGTA) had no effect on swelling-induced taurine release but prevented ATP-induced stimulation. H 2 O 2 , ATP, and ionomycin were unable to stimulate the taurine release in the presence of AA861 or Zafirlukast, placing 5-LO and CysLT 1 as essential elements in the swelling-induced activation of VSOAC with ROS and Ca 2+ as potent modulators. Inhibition of tyrosine kinases (genistein, cucurbitacin) reduces volume-sensitive taurine release, adding tyrosine kinases (Janus kinase) as regulators of VSOAC activity. Caspase-3 activity during hypoxia is unaffected by inhibition of 5-LO/CysLT 1 but reduced when swelling-induced taurine loss via VSOAC is prevented by DIDS excess extracellular taurine, indicating a beneficial role of taurine under hypoxia." @default.
- W2011346118 created "2016-06-24" @default.
- W2011346118 creator A5053059598 @default.
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- W2011346118 date "2013-07-01" @default.
- W2011346118 modified "2023-09-25" @default.
- W2011346118 title "Volume-sensitive release of organic osmolytes in the human lung epithelial cell line A549: role of the 5-lipoxygenase" @default.
- W2011346118 cites W1515653072 @default.
- W2011346118 cites W1528616059 @default.
- W2011346118 cites W1580439837 @default.
- W2011346118 cites W1969976873 @default.
- W2011346118 cites W1973020874 @default.
- W2011346118 cites W1978539396 @default.
- W2011346118 cites W1980153781 @default.
- W2011346118 cites W1992193255 @default.
- W2011346118 cites W1993209206 @default.
- W2011346118 cites W1995065584 @default.
- W2011346118 cites W1998925732 @default.
- W2011346118 cites W2006795881 @default.
- W2011346118 cites W2009367981 @default.
- W2011346118 cites W2012886608 @default.
- W2011346118 cites W2013795470 @default.
- W2011346118 cites W2014169018 @default.
- W2011346118 cites W2014724038 @default.
- W2011346118 cites W2017376578 @default.
- W2011346118 cites W2018430618 @default.
- W2011346118 cites W2023842030 @default.
- W2011346118 cites W2024141104 @default.
- W2011346118 cites W2029729156 @default.
- W2011346118 cites W2032440198 @default.
- W2011346118 cites W2032586838 @default.
- W2011346118 cites W2035043164 @default.
- W2011346118 cites W2040397458 @default.
- W2011346118 cites W2041310139 @default.
- W2011346118 cites W2046892943 @default.
- W2011346118 cites W2047112821 @default.
- W2011346118 cites W2051569936 @default.
- W2011346118 cites W2054318930 @default.
- W2011346118 cites W2054780906 @default.
- W2011346118 cites W2060818119 @default.
- W2011346118 cites W2061253319 @default.
- W2011346118 cites W2061969522 @default.
- W2011346118 cites W2062759956 @default.
- W2011346118 cites W2063280195 @default.
- W2011346118 cites W2064617184 @default.
- W2011346118 cites W2067060142 @default.
- W2011346118 cites W2070649129 @default.
- W2011346118 cites W2071029414 @default.
- W2011346118 cites W2076090601 @default.
- W2011346118 cites W2078314973 @default.
- W2011346118 cites W2081297019 @default.
- W2011346118 cites W2083111365 @default.
- W2011346118 cites W2088254327 @default.
- W2011346118 cites W2092049631 @default.
- W2011346118 cites W2093785349 @default.
- W2011346118 cites W2094358674 @default.
- W2011346118 cites W2098959380 @default.
- W2011346118 cites W2102136911 @default.
- W2011346118 cites W2107014409 @default.
- W2011346118 cites W2111761608 @default.
- W2011346118 cites W2116250583 @default.
- W2011346118 cites W2118444090 @default.
- W2011346118 cites W2121584076 @default.
- W2011346118 cites W2123758719 @default.
- W2011346118 cites W2125588561 @default.
- W2011346118 cites W2132319231 @default.
- W2011346118 cites W2134530864 @default.
- W2011346118 cites W2139703860 @default.
- W2011346118 cites W2140353112 @default.
- W2011346118 cites W2151487527 @default.
- W2011346118 cites W2154592130 @default.
- W2011346118 cites W2156132591 @default.
- W2011346118 cites W2170875784 @default.
- W2011346118 cites W2177909750 @default.
- W2011346118 cites W2323423108 @default.
- W2011346118 cites W2412043390 @default.
- W2011346118 cites W2414745270 @default.
- W2011346118 cites W24526873 @default.
- W2011346118 cites W24673175 @default.
- W2011346118 cites W98155210 @default.
- W2011346118 doi "https://doi.org/10.1152/ajpcell.00412.2012" @default.
- W2011346118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23485709" @default.
- W2011346118 hasPublicationYear "2013" @default.
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