FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011371298> ?p ?o ?g. }

• W2011371298 endingPage "259" @default.
• W2011371298 startingPage "247" @default.
• W2011371298 abstract "Although the product of the UL12 gene of herpes simplex virus type 1 (HSV-1) has been shown to possess both exonuclease and endonuclease activitiesin vitro,and deletion of most of the gene within the viral genome results in inefficient production and maturation of infectious virions, the function of the deoxyribonuclease (DNase) activity per se in virus replication remains unclear. In order to correlate thein vitroandin vivoactivities of the protein encoded by UL12, mutant proteins were tested for nuclease activityin vitroby a novel hypersensitivity cleavage assay and for their ability to complement the replication of a DNase null mutant, AN-1. Rabbit reticulocyte lysates programmed with wild-type UL12 RNA cleaved at the same sites cleaved by purified HSV-1 DNase, but distinct from those cleaved by DNase I or micrococcal nuclease. All mutants which lacked DNase activityin vitroalso failed to complement the replication of AN-1 in nonpermissive cells. Likewise, all mutants which contained HSV-1 DNase activity, as detected by the hypersensitivity cleavage assay, were capable of complementing the replication of the DNase null mutant, though to varying extents. Of particular note was the d1-126 mutant protein, which, despite having the same specific activity as the wild-type enzymein vitro,complemented the replication of AN-1 significantly less than the wild-type protein. The results suggest that DNase activity per se is required for efficient replication of HSV-1in vivo.However, residues, including the N-terminal 126 amino acids, which are dispensable for enzymatic activityin vitromay facilitate the accessibility or activity of the proteinin vivo." @default.
• W2011371298 created "2016-06-24" @default.
• W2011371298 creator A5008513787 @default.
• W2011371298 creator A5036498984 @default.
• W2011371298 creator A5051476286 @default.
• W2011371298 date "1998-03-01" @default.
• W2011371298 modified "2023-09-27" @default.
• W2011371298 title "Structure–Function Analysis of the Herpes Simplex Virus Type 1 UL12 Gene: Correlation of Deoxyribonuclease Activityin Vitrowith Replication Function" @default.
• W2011371298 cites W1484824530 @default.
• W2011371298 cites W1487145741 @default.
• W2011371298 cites W1492788909 @default.
• W2011371298 cites W1501357974 @default.
• W2011371298 cites W1505916481 @default.
• W2011371298 cites W1521504922 @default.
• W2011371298 cites W1581400567 @default.
• W2011371298 cites W1584221034 @default.
• W2011371298 cites W1596194460 @default.
• W2011371298 cites W1613555203 @default.
• W2011371298 cites W1747422055 @default.
• W2011371298 cites W1852387875 @default.
• W2011371298 cites W1891768184 @default.
• W2011371298 cites W1905249227 @default.
• W2011371298 cites W1971403634 @default.
• W2011371298 cites W1979775862 @default.
• W2011371298 cites W1987144751 @default.
• W2011371298 cites W1994930386 @default.
• W2011371298 cites W2005524609 @default.
• W2011371298 cites W2032527591 @default.
• W2011371298 cites W2040242075 @default.
• W2011371298 cites W2040441008 @default.
• W2011371298 cites W2040575169 @default.
• W2011371298 cites W2051353838 @default.
• W2011371298 cites W2055898827 @default.
• W2011371298 cites W2056650797 @default.
• W2011371298 cites W2056730104 @default.
• W2011371298 cites W2076024234 @default.
• W2011371298 cites W2095333277 @default.
• W2011371298 cites W2101108802 @default.
• W2011371298 cites W2112805999 @default.
• W2011371298 cites W2116525679 @default.
• W2011371298 cites W2118773705 @default.
• W2011371298 cites W2132288616 @default.
• W2011371298 cites W2135250095 @default.
• W2011371298 cites W2135972272 @default.
• W2011371298 cites W2158953266 @default.
• W2011371298 cites W2333689301 @default.
• W2011371298 doi "https://doi.org/10.1006/viro.1998.9054" @default.
• W2011371298 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9527934" @default.
• W2011371298 hasPublicationYear "1998" @default.
• W2011371298 type Work @default.
• W2011371298 sameAs 2011371298 @default.
• W2011371298 citedByCount "19" @default.
• W2011371298 countsByYear W20113712982012 @default.
• W2011371298 countsByYear W20113712982013 @default.
• W2011371298 countsByYear W20113712982014 @default.
• W2011371298 countsByYear W20113712982015 @default.
• W2011371298 countsByYear W20113712982017 @default.
• W2011371298 countsByYear W20113712982019 @default.
• W2011371298 countsByYear W20113712982020 @default.
• W2011371298 crossrefType "journal-article" @default.
• W2011371298 hasAuthorship W2011371298A5008513787 @default.
• W2011371298 hasAuthorship W2011371298A5036498984 @default.
• W2011371298 hasAuthorship W2011371298A5051476286 @default.
• W2011371298 hasBestOaLocation W20113712981 @default.
• W2011371298 hasConcept C104317684 @default.
• W2011371298 hasConcept C139024627 @default.
• W2011371298 hasConcept C140704245 @default.
• W2011371298 hasConcept C143065580 @default.
• W2011371298 hasConcept C153911025 @default.
• W2011371298 hasConcept C159047783 @default.
• W2011371298 hasConcept C182614070 @default.
• W2011371298 hasConcept C2522874641 @default.
• W2011371298 hasConcept C2777028655 @default.
• W2011371298 hasConcept C2777271071 @default.
• W2011371298 hasConcept C2779377703 @default.
• W2011371298 hasConcept C2781196997 @default.
• W2011371298 hasConcept C3017666073 @default.
• W2011371298 hasConcept C55493867 @default.
• W2011371298 hasConcept C58237431 @default.
• W2011371298 hasConcept C73573662 @default.
• W2011371298 hasConcept C83640560 @default.
• W2011371298 hasConcept C84772758 @default.
• W2011371298 hasConcept C86803240 @default.
• W2011371298 hasConceptScore W2011371298C104317684 @default.
• W2011371298 hasConceptScore W2011371298C139024627 @default.
• W2011371298 hasConceptScore W2011371298C140704245 @default.
• W2011371298 hasConceptScore W2011371298C143065580 @default.
• W2011371298 hasConceptScore W2011371298C153911025 @default.
• W2011371298 hasConceptScore W2011371298C159047783 @default.
• W2011371298 hasConceptScore W2011371298C182614070 @default.
• W2011371298 hasConceptScore W2011371298C2522874641 @default.
• W2011371298 hasConceptScore W2011371298C2777028655 @default.
• W2011371298 hasConceptScore W2011371298C2777271071 @default.
• W2011371298 hasConceptScore W2011371298C2779377703 @default.
• W2011371298 hasConceptScore W2011371298C2781196997 @default.
• W2011371298 hasConceptScore W2011371298C3017666073 @default.
• W2011371298 hasConceptScore W2011371298C55493867 @default.
• W2011371298 hasConceptScore W2011371298C58237431 @default.

• next