FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011377754> ?p ?o ?g. }

• W2011377754 endingPage "2856" @default.
• W2011377754 startingPage "2849" @default.
• W2011377754 abstract "Growth differentiation factor-9 (GDF-9), a member of the transforming growth factor superfamily, modulates the development and function of granulosa and theca cells. Targeted deletion of GDF-9 in the mouse revealed that GDF-9 was essential for the establishment of the thecal cell layer during early folliculogenesis. During later stages of follicular development, the roles of GDF-9 are less well understood, but it has been postulated that oocyte-derived GDF-9 may prevent premature luteinization of follicular cells, based on its ability to modulate steroidogenesis by rodent ovarian cells. In the rodent, GDF-9 is expressed solely by the oocyte from the early primary follicular stage through ovulation. Recent studies in the rhesus monkey demonstrated that granulosa cells express GDF-9, suggesting a broader role for this protein in ovarian function in primates. We examined the effect of recombinant GDF-9 on proliferating human granulosa and thecal cell steroidogenesis and the expression of steroidogenic acute regulatory protein (StAR), P450 side-chain cleavage, and P450 aromatase. We also examined granulosa cell GDF-9 expression by quantitative RT-PCR and by Western analysis. GDF-9 inhibited 8-Br-cAMP-stimulated granulosa progesterone synthesis by approximately 40%, but did not affect basal progesterone production. Concordant with reduced steroid production, 8-Br-cAMP-stimulated StAR protein expression was reduced approximately 40% in granulosa cells, as were expression of StAR mRNA and StAR promoter activity. Additionally, GDF-9 inhibited 8-Br-cAMP-stimulated expression of P450 side-chain cleavage and P450 aromatase. Human granulosa cells expressed GDF-9, as determined by RT-PCR and Western analysis. Treatment of human thecal cells with GDF-9 blocked forskolin-stimulated progesterone, 17alpha-hydroxyprogesterone, and dehydroepiandrosterone synthesis. Thecal cells exhibited greater sensitivity to GDF-9, suggesting that this cell may be a primary target of GDF-9. Moreover, GDF-9 increased thecal cell numbers during culture, but had no effect on granulosa cell growth. Our findings implicate GDF-9 in the modulation of follicular steroidogenesis, especially theca cell function. Because GDF-9 mRNA and protein are detectable in granulosa-lutein cells after the LH surge, the concept of GDF-9 as a solely oocyte-derived luteinization inhibitor needs to be reevaluated." @default.
• W2011377754 created "2016-06-24" @default.
• W2011377754 creator A5030631296 @default.
• W2011377754 creator A5032191719 @default.
• W2011377754 creator A5048183362 @default.
• W2011377754 creator A5090491933 @default.
• W2011377754 date "2002-06-01" @default.
• W2011377754 modified "2023-10-16" @default.
• W2011377754 title "Growth Differentiation Factor-9 Inhibits 3′5′-Adenosine Monophosphate-Stimulated Steroidogenesis in Human Granulosa and Theca Cells" @default.
• W2011377754 cites W1973628165 @default.
• W2011377754 cites W1973841249 @default.
• W2011377754 cites W1981538206 @default.
• W2011377754 cites W1995586478 @default.
• W2011377754 cites W1996938657 @default.
• W2011377754 cites W2003432885 @default.
• W2011377754 cites W2005444928 @default.
• W2011377754 cites W2009443375 @default.
• W2011377754 cites W2018831893 @default.
• W2011377754 cites W2019771407 @default.
• W2011377754 cites W2035048391 @default.
• W2011377754 cites W2036334672 @default.
• W2011377754 cites W2037791269 @default.
• W2011377754 cites W2040525542 @default.
• W2011377754 cites W2041944050 @default.
• W2011377754 cites W2049004724 @default.
• W2011377754 cites W2055314203 @default.
• W2011377754 cites W2057363866 @default.
• W2011377754 cites W2069466619 @default.
• W2011377754 cites W2071629674 @default.
• W2011377754 cites W2075107566 @default.
• W2011377754 cites W2077861288 @default.
• W2011377754 cites W2089269914 @default.
• W2011377754 cites W2107096204 @default.
• W2011377754 cites W2111508487 @default.
• W2011377754 cites W2113746705 @default.
• W2011377754 cites W2127208573 @default.
• W2011377754 cites W2129225495 @default.
• W2011377754 cites W2143877282 @default.
• W2011377754 cites W2147322409 @default.
• W2011377754 cites W2160616140 @default.
• W2011377754 cites W2162233013 @default.
• W2011377754 cites W2166932843 @default.
• W2011377754 cites W2409639199 @default.
• W2011377754 doi "https://doi.org/10.1210/jcem.87.6.8551" @default.
• W2011377754 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12050262" @default.
• W2011377754 hasPublicationYear "2002" @default.
• W2011377754 type Work @default.
• W2011377754 sameAs 2011377754 @default.
• W2011377754 citedByCount "32" @default.
• W2011377754 countsByYear W20113777542012 @default.
• W2011377754 countsByYear W20113777542013 @default.
• W2011377754 countsByYear W20113777542014 @default.
• W2011377754 countsByYear W20113777542015 @default.
• W2011377754 countsByYear W20113777542016 @default.
• W2011377754 countsByYear W20113777542017 @default.
• W2011377754 countsByYear W20113777542018 @default.
• W2011377754 countsByYear W20113777542020 @default.
• W2011377754 countsByYear W20113777542021 @default.
• W2011377754 crossrefType "journal-article" @default.
• W2011377754 hasAuthorship W2011377754A5030631296 @default.
• W2011377754 hasAuthorship W2011377754A5032191719 @default.
• W2011377754 hasAuthorship W2011377754A5048183362 @default.
• W2011377754 hasAuthorship W2011377754A5090491933 @default.
• W2011377754 hasBestOaLocation W20113777541 @default.
• W2011377754 hasConcept C104317684 @default.
• W2011377754 hasConcept C121608353 @default.
• W2011377754 hasConcept C126322002 @default.
• W2011377754 hasConcept C134018914 @default.
• W2011377754 hasConcept C150194340 @default.
• W2011377754 hasConcept C175805485 @default.
• W2011377754 hasConcept C179933525 @default.
• W2011377754 hasConcept C187785154 @default.
• W2011377754 hasConcept C196843134 @default.
• W2011377754 hasConcept C2776166826 @default.
• W2011377754 hasConcept C2777203538 @default.
• W2011377754 hasConcept C2779192323 @default.
• W2011377754 hasConcept C2779670467 @default.
• W2011377754 hasConcept C2780536345 @default.
• W2011377754 hasConcept C530470458 @default.
• W2011377754 hasConcept C5512455 @default.
• W2011377754 hasConcept C55493867 @default.
• W2011377754 hasConcept C71924100 @default.
• W2011377754 hasConcept C86803240 @default.
• W2011377754 hasConcept C95444343 @default.
• W2011377754 hasConceptScore W2011377754C104317684 @default.
• W2011377754 hasConceptScore W2011377754C121608353 @default.
• W2011377754 hasConceptScore W2011377754C126322002 @default.
• W2011377754 hasConceptScore W2011377754C134018914 @default.
• W2011377754 hasConceptScore W2011377754C150194340 @default.
• W2011377754 hasConceptScore W2011377754C175805485 @default.
• W2011377754 hasConceptScore W2011377754C179933525 @default.
• W2011377754 hasConceptScore W2011377754C187785154 @default.
• W2011377754 hasConceptScore W2011377754C196843134 @default.
• W2011377754 hasConceptScore W2011377754C2776166826 @default.
• W2011377754 hasConceptScore W2011377754C2777203538 @default.
• W2011377754 hasConceptScore W2011377754C2779192323 @default.
• W2011377754 hasConceptScore W2011377754C2779670467 @default.
• W2011377754 hasConceptScore W2011377754C2780536345 @default.

• next