Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011393766> ?p ?o ?g. }
- W2011393766 endingPage "589" @default.
- W2011393766 startingPage "579" @default.
- W2011393766 abstract "Fertilization of sea urchin eggs results in a large, transient increase in intracellular free Ca2+ concentration that is responsible for re-initiation of the cell division cycle. We show that activation of ERK1, a Ca2+-dependent MAP kinase response, is required for both DNA synthesis and cell cycle progression after fertilization. We combine experiments on populations of cells with analysis at the single cell level, and develop a proxy assay for DNA synthesis in single embryos, using GFP-PCNA. We compare the effects of low molecular weight inhibitors with a recombinant approach targeting the same signalling pathway. We find that inhibition of the ERK pathway at fertilization using either recombinant ERK phosphatase or U0126, a MEK inhibitor, prevents accumulation of GFP-PCNA in the zygote nucleus and that U0126 prevents incorporation of [3H]-thymidine into DNA. Abrogation of the ERK1 signalling pathway also prevents chromatin decondensation of the sperm chromatin after pronuclear fusion, nuclear envelope breakdown and formation of a bipolar spindle." @default.
- W2011393766 created "2016-06-24" @default.
- W2011393766 creator A5027773946 @default.
- W2011393766 creator A5029592071 @default.
- W2011393766 creator A5036838965 @default.
- W2011393766 creator A5054995174 @default.
- W2011393766 creator A5080674612 @default.
- W2011393766 creator A5088157413 @default.
- W2011393766 date "2005-02-01" @default.
- W2011393766 modified "2023-09-26" @default.
- W2011393766 title "ERK1 activation is required for S-phase onset and cell cycle progression after fertilization in sea urchin embryos" @default.
- W2011393766 cites W1522823874 @default.
- W2011393766 cites W1581417930 @default.
- W2011393766 cites W1778387444 @default.
- W2011393766 cites W1807534989 @default.
- W2011393766 cites W1911421926 @default.
- W2011393766 cites W1946343960 @default.
- W2011393766 cites W1965390803 @default.
- W2011393766 cites W1966199536 @default.
- W2011393766 cites W1966260349 @default.
- W2011393766 cites W1966550437 @default.
- W2011393766 cites W1968201777 @default.
- W2011393766 cites W1976397353 @default.
- W2011393766 cites W1979915283 @default.
- W2011393766 cites W1987246052 @default.
- W2011393766 cites W1989730199 @default.
- W2011393766 cites W1995254753 @default.
- W2011393766 cites W2001199959 @default.
- W2011393766 cites W2004993580 @default.
- W2011393766 cites W2006730617 @default.
- W2011393766 cites W2011928555 @default.
- W2011393766 cites W2013153244 @default.
- W2011393766 cites W2035693310 @default.
- W2011393766 cites W2037426608 @default.
- W2011393766 cites W2038447145 @default.
- W2011393766 cites W2039524691 @default.
- W2011393766 cites W2041254889 @default.
- W2011393766 cites W2044071398 @default.
- W2011393766 cites W2044884232 @default.
- W2011393766 cites W2046277481 @default.
- W2011393766 cites W2080987500 @default.
- W2011393766 cites W2082723977 @default.
- W2011393766 cites W2086262311 @default.
- W2011393766 cites W2089448004 @default.
- W2011393766 cites W2091092785 @default.
- W2011393766 cites W2091353256 @default.
- W2011393766 cites W2104764445 @default.
- W2011393766 cites W2108862848 @default.
- W2011393766 cites W2109623711 @default.
- W2011393766 cites W2126122106 @default.
- W2011393766 cites W2131739843 @default.
- W2011393766 cites W2136630749 @default.
- W2011393766 cites W2158811212 @default.
- W2011393766 cites W2160024787 @default.
- W2011393766 cites W2162692975 @default.
- W2011393766 cites W2163751122 @default.
- W2011393766 cites W2167211051 @default.
- W2011393766 cites W2186711421 @default.
- W2011393766 cites W2337853871 @default.
- W2011393766 cites W2410629393 @default.
- W2011393766 cites W2413988515 @default.
- W2011393766 cites W4238527967 @default.
- W2011393766 doi "https://doi.org/10.1242/dev.01607" @default.
- W2011393766 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15634691" @default.
- W2011393766 hasPublicationYear "2005" @default.
- W2011393766 type Work @default.
- W2011393766 sameAs 2011393766 @default.
- W2011393766 citedByCount "22" @default.
- W2011393766 countsByYear W20113937662012 @default.
- W2011393766 countsByYear W20113937662013 @default.
- W2011393766 countsByYear W20113937662014 @default.
- W2011393766 countsByYear W20113937662015 @default.
- W2011393766 crossrefType "journal-article" @default.
- W2011393766 hasAuthorship W2011393766A5027773946 @default.
- W2011393766 hasAuthorship W2011393766A5029592071 @default.
- W2011393766 hasAuthorship W2011393766A5036838965 @default.
- W2011393766 hasAuthorship W2011393766A5054995174 @default.
- W2011393766 hasAuthorship W2011393766A5080674612 @default.
- W2011393766 hasAuthorship W2011393766A5088157413 @default.
- W2011393766 hasBestOaLocation W20113937661 @default.
- W2011393766 hasConcept C120504264 @default.
- W2011393766 hasConcept C1491633281 @default.
- W2011393766 hasConcept C153911025 @default.
- W2011393766 hasConcept C184235292 @default.
- W2011393766 hasConcept C196843134 @default.
- W2011393766 hasConcept C2776908312 @default.
- W2011393766 hasConcept C28748434 @default.
- W2011393766 hasConcept C29537977 @default.
- W2011393766 hasConcept C34688535 @default.
- W2011393766 hasConcept C54355233 @default.
- W2011393766 hasConcept C552990157 @default.
- W2011393766 hasConcept C55493867 @default.
- W2011393766 hasConcept C57074206 @default.
- W2011393766 hasConcept C62112901 @default.
- W2011393766 hasConcept C6902291 @default.
- W2011393766 hasConcept C83640560 @default.
- W2011393766 hasConcept C85813293 @default.
- W2011393766 hasConcept C86803240 @default.