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- W2011397327 abstract "A molecule with an anisotropic magnetic susceptibility is spontaneously aligned in a static magnetic field. Alignment of such a molecule yields residual dipolar couplings and pseudocontact shifts. Lanthanide ions have recently been successfully used to provide an anisotropic magnetic susceptibility in target molecules either by replacing a calcium ion with a lanthanide ion in calcium-binding proteins or by attaching an EDTA derivative to a cysteine residue via a disulfide bond. Here we describe a novel enantiomerically pure EDTA derived tag that aligns stronger due to its shorter linker and does not suffer from stereochemical diversity upon lanthanide complexation. We observed residual 15N,1H-dipolar couplings of up to 8 Hz at 800 MHz induced by a single alignment tensor from this tag." @default.
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- W2011397327 date "2004-07-01" @default.
- W2011397327 modified "2023-10-16" @default.
- W2011397327 title "Novel Techniques for Weak Alignment of Proteins in Solution Using Chemical Tags Coordinating Lanthanide Ions" @default.
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- W2011397327 doi "https://doi.org/10.1023/b:jnmr.0000032611.72827.de" @default.
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