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• W2011457585 abstract "The interaction of βIII-depleted tubulin with taxol was investigated. A monoclonal antibody against the βIII tubulin isotype was immobilized on a sepharose 4B column and used to remove the βIII tubulin isotype from unfractionated tubulin. The assembly of βIII-depleted tubulin in the presence of taxol was enhanced compared to unfractionated tubulin. The critical concentration of unfractionated tubulin in the presence of 10 μM taxol is 0.4 mg/ml, while the critical concentration of βIII-depleted tubulin is 0.16 mg/ml. At different concentrations of taxol, the assembly of βIII-depleted tubulin is increased relative to that of un fractionated tubulin and reaches the maximumat about a 1 : 1 ratio of tubulin and taxol. The assembly of un fractionated tubulin and βIII-depleted tubulin has also been studied by electron microscopy. After 2 minutes at 37°C, unfractionated tubulin assembly in the presence of 10 μM taxol results only in ribbon-like and ring structures; there are no visible microtubules. By 5 minutes, microtubules appear and increase in length. The assembly of βIII-depleted tubulin in the presence of 10 μM taxol occurs more quickly. In contrast to the case with un fractionated tubulin, βIII-depleted tubulin assembles within 2 minutes into microtubules which increase in length with time. At 30 minutes, microtubules assembled from βIII-depleted tubulin are shorter than the microtubules assembled from unfractionated tubulin. There is no visible difference between the microtubules assembled from unfractionated tubulin and βIII-depleted tubulin. Taxol-induced βIII-depleted tubulin assembly is more resistant to the inhibiting effect of podophyllotoxin and colchicine. It is also less sensitive to the inhibiting effect of cold temperature." @default.
• W2011457585 created "2016-06-24" @default.
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• W2011457585 date "1993-01-01" @default.
• W2011457585 modified "2023-10-01" @default.
• W2011457585 title "Removal of .BETA.III Isotype Enhances Taxol Induced Microtubule Assembly." @default.
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• W2011457585 doi "https://doi.org/10.1247/csf.18.173" @default.
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