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- W2011492525 abstract "ABSTRACT In McA-RH 8994 rat hepatoma cells, all- trans retinoic acid ( t -RA) induces expression of the α-fetoprotein (AFP) and albumin genes and results in a phenotype similar to differentiated fetal hepatocytes. The present study elucidated the mechanism involved in AFP gene regulation mediated by retinoic acid. Northern blot analyses demonstrated that 9- cis -retinoic acid ( c -RA), a ligand for retinoid x receptors (RXRs), also induced expression of the AFP gene in McA-RH 8994 cells. The induction was time- and dose-dependent. Northern blots and transfection assays using the 7·3 kb full-length regulatory region of the AFP gene demonstrated that c -RA was more effective than t -RA in regulating expression of the AFP gene. At 10 −7 m , c -RA increased AFP mRNA 5-fold and chloramphenicol acetyltransferase (CAT) activity 2·5-fold. In contrast, t -RA at a concentration of 10 −7 m exerted no significant effect; 10 − 6 to 10 −5 m t -RA was needed to affect AFP gene expression. These data suggested that activation of RXRs is essential for the regulation of the AFP gene. Co-transfection experiments revealed that overexpression of RXRα in McA-RH 8994 cells further enhanced the CAT activity induced by c -RA. In addition, c -RA did not alter the half-life of AFP mRNA. Thus, RXRα may play a crucial role in transcriptional regulation of the AFP gene and in controlling hepatocyte phenotype." @default.
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- W2011492525 date "1995-02-01" @default.
- W2011492525 modified "2023-09-26" @default.
- W2011492525 title "9-cis-retinoic acid is more effective than all-trans-retinoic acid in upregulating expression of the α-fetoprotein gene" @default.
- W2011492525 doi "https://doi.org/10.1677/jme.0.0140101" @default.
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