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- W2011495282 abstract "The use of β-glucuronidase (β-GUS) as a reporter and sensitive detection system for Yarrowia lipolytica was studied. The Escherichia coli gusA gene was expressed under control of the homologous LEU2 promoter in a transcriptional fusion. An NcoI restriction site was introduced at the translational start-ATG, conserving the most favorable context for initiation of translation. The chimeric LEU2′-gusA gene was integrated into the LEU2 locus by homologous recombination. The β-GUS assay was very sensitive and highly reproducible, using the cytosolic fraction or a total cell extract as the source of enzyme. In a leucine-free medium, β-GUS activity was at a high, constant level, independent of growth phase. In transformants grown on complete medium, β-GUS activity was reduced about three-fold, but doubled during logarithmic growth. No intrinsic β-GUS activity was detectable in untransformed Y. lipolytica and no effect of β-GUS expression on growth was obseved. β-GUS-producing Y. lipolytica cells could be directly detected on media plates containing X-gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronide)." @default.
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- W2011495282 date "1993-01-01" @default.
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- W2011495282 title "Functional expression of bacterial β-glucuronidase and its use as a reporter system in the yeastYarrowia lipolytica" @default.
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- W2011495282 doi "https://doi.org/10.1002/yea.320090109" @default.
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