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• W2011602893 abstract "Mass transfer limitations of scaffolds are currently hindering the development of 3-dimensional, clinically viable, tissue engineered constructs. We have developed a poly(lactide-co-glycolide) (PLGA) hollow fibre membrane scaffold that will provide support for cell culture, allow psuedovascularisation in vitro and provide channels for angiogenesis in vivo. We produced PDLLGA flat sheet membranes using 1, 4-dioxane and 1-methyl-2-pyrrolidinone (NMP) as solvents and water as the nonsolvent, and hollow fibre membranes, using NMP and water, by dry/wet- and wet-spinning. The resulting fibres had an outer diameter of 700 μm and an inner diameter of 250 μm with 0.2–1.0 μm pores on the culture surface. It was shown that varying the air gap and temperature when spinning changed the morphology of the fibres. The introduction of a 50 mm air gap caused a dense skin of 5 μm thick to form, compared to a skin of 0.5 μm thick without an air gap. Spinning at 40°C produced fibres with a more open central section in the wall that contained more, larger macrovoids compared to fibres spun at 20°C. Culture of the immortalised osteogenic cell line 560pZIPv.neo (pZIP) was carried out on the PDLLGA flat sheets in static culture and in a PDLLGA hollow fibre bioreactor under counter-current flow conditions. Attachment and proliferation was statistically similar to tissue culture polystyrene on the flat sheets and was also successful in the hollow fibre bioreactor. The PDLLGA hollow fibres are a promising scaffold to address the size limitations currently seen in tissue engineered constructs. Biotechnol. Bioeng. 2007;96: 177–187. © 2006 Wiley Periodicals, Inc." @default.
• W2011602893 created "2016-06-24" @default.
• W2011602893 creator A5010881984 @default.
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• W2011602893 date "2006-01-01" @default.
• W2011602893 modified "2023-10-02" @default.
• W2011602893 title "Poly(lactic-co-glycolic acid) hollow fibre membranes for use as a tissue engineering scaffold" @default.
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• W2011602893 doi "https://doi.org/10.1002/bit.21093" @default.
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