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• W2011705087 abstract "Purpose: The shortage of donor livers for hepatocyte isolation is one of the major limiting factors for bioartificial liver (BAL) therapy. The present study describes a novel method for immortalizing healthy human hepatocytes as a source in BAL. Methods: Human hepatocytes were transduced with a retroviral vector encoding the genes of telomerase reverse transcriptase (hTERT) and positive selectable marker GFP which were flanked by a pair of recombination target loxP. After the transduction of hepatocytes with SSR#197, cells were processed by FACS to obtain GFP-positive populations. The gene expression of differentiated liver functions and efficacy of Cre/loxP recombination mediated by an adenoviral vector producing Cre (Ad-Cre) were evaluated in SSR#197-transduced hepatocytes. Results: TTNT-1, one of SSR#197-transduced hepatocytes followed by sorting for GFP, grew steadily in tissue culture and demonstrated morphological characteristics of liver parenchymal cells. Efficient Cre/loxP recombination in TTNT-1 cells was performed following Ad-Cre infection and subsequent cell sorting for GFP-negative population. After recombination, glycogen synthesis and gene expression of differentiated liver functions increased. Conclusion: We have developed a novel method of reversible immortalization in human hepatocytes. This work represents a potentially novel strategy for addressing the problem of organ shortage that currently limits the use of HTX." @default.
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• W2011705087 date "2001-03-01" @default.
• W2011705087 modified "2023-09-25" @default.
• W2011705087 title "hTERT-TRANSDUCED HUMAN HEPATOCYTES AS A SOURCE OF A BIOARTIFICIAL LIVER" @default.
• W2011705087 doi "https://doi.org/10.1097/00002480-200103000-00257" @default.
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